This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lund, A Articles by Vartdal, F Search for Related Content PubMed PubMed Citation Articles by Lund, A Articles by Vartdal, F

 Previous Article  |  Next Article 

J Clin Microbiol. 1988 December; 26(12): 2572-2575

Rapid isolation of K88+ Escherichia coli by using immunomagnetic particles.

National Veterinary Institute, Oslo, Norway.

ABSTRACT

Superparamagnetic polymer particles precoated with sheep anti-mouse immunoglobulin G were coated with immunoglobulin G2 monoclonal antibodies to the K88 antigen of Escherichia coli (MAb-K88). These immunomagnetic particles (IMP) were used for isolation and identification of K88 antigen-positive (K88+) E. coli. The bacteria presenting the K88 antigen were easily isolated in almost pure culture from a mixed culture of five different O serogroups of E. coli. Nonspecific binding of K88 antigen-negative (K88-) E. coli to the IMP was not observed. The sensitivity of the test to detect K88+ E. coli was found to be 4,000 CFU/ml with fluorescence microscopy. When bacteria attached to the MAb-K88 IMP were grown on blood agar, about 20% of the initial number of CFU was recovered. The test is promising as a rapid method for isolation and identification of K88+ E. coli from a mixed culture.

This article has been cited by other articles:

• Koo, K., Foegeding, P. M., Swaisgood, H. E. (1998). Development of a Streptavidin-Conjugated Single-Chain Antibody That Binds Bacillus cereus Spores. Appl. Environ. Microbiol. 64: 2497-2502 [Abstract] [Full Text]  
• Gagne, A, Lacouture, S, Broes, A, D'Allaire, S, Gottschalk, M (1998). Development of an immunomagnetic method for selective isolation of Actinobacillus pleuropneumoniae serotype 1 from tonsils [In Process Citation]. J. Clin. Microbiol. 36: 251-254 [Abstract] [Full Text]