This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chou, S W Articles by Scott, K M Search for Related Content PubMed PubMed Citation Articles by Chou, S W Articles by Scott, K M

Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein.

Medical Service, Veterans Administration Medical Center, Portland, Oregon.

ABSTRACT

An overnight assay, based on staining cytomegalovirus-infected cells with monoclonal antibody to the 72,000-molecular-weight major immediate-early viral protein, was compared with a conventional 14-day plaque assay for quantitation of cell-free stocks of cytomegalovirus laboratory strain AD-169 and 20 other clinical strains. Viral titers were quantitatively similar when determined by either method, but centrifugation of monolayers during inoculation enhanced viral infectivity an average of 4.1-fold. When used for scoring neutralizing antibody assays, monoclonal antibody staining yielded titers within one dilution of 14-day plaque-reduction assays in 54 of 56 titrations. Of 21 cytomegalovirus strains, 2 were not recognized by the monoclonal antibody used. Assay with monoclonal antibody offers a rapid and accurate alternative to plaque assays for quantitation or neutralization of cytomegalovirus.

This article has been cited by other articles:

• Ueno, T., Eizuru, Y., Katano, H., Kurata, T., Sata, T., Irie, S., Ogawa-Goto, K. (2006). Novel Real-Time Monitoring System for Human Cytomegalovirus- Infected Cells In Vitro That Uses a Green Fluorescent Protein-PML-Expressing Cell Line.. Antimicrob. Agents Chemother. 50: 2806-2813 [Abstract] [Full Text]  
• Utama, B., Shen, Y. H., Mitchell, B. M., Makagiansar, I. T., Gan, Y., Muthuswamy, R., Duraisamy, S., Martin, D., Wang, X., Zhang, M.-X., Wang, J., Wang, J., Vercellotti, G. M., Gu, W., Li Wang, X. (2006). Mechanisms for human cytomegalovirus-induced cytoplasmic p53 sequestration in endothelial cells. J. Cell Sci. 119: 2457-2467 [Abstract] [Full Text]  
• Sia, I. G., Patel, R. (2000). New Strategies for Prevention and Therapy of Cytomegalovirus Infection and Disease in Solid-Organ Transplant Recipients. Clin. Microbiol. Rev. 13: 83-121 [Abstract] [Full Text]  
• Boeckh, M., Boivin, G. (1998). Quantitation of Cytomegalovirus: Methodologic Aspects and Clinical Applications. Clin. Microbiol. Rev. 11: 533-554 [Abstract] [Full Text]