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Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera.

Department of Laboratory Medicine, University of Washington, Seattle 98105.

ABSTRACT

Sera from patients with culture-proven genital herpes infections were tested for herpes simplex virus type 1 (HSV-1)- and HSV-2-specific antibodies by both a Western blot (immunoblot) technique (WBA) and immunodot enzyme assays (IEAs) specific for HSV-1 or HSV-2 glycoprotein G (gG). Of 137 serum samples tested, none was mistyped by either WBA or IEA. Both tests were most sensitive with sera obtained at least 21 days after onset of primary HSV-2 infections or sera drawn during recurrent HSV-2 genital episodes: 75 of 76 (99%) such serum samples were positive for HSV-2 antibody by WBA and 73 of 76 (96%) were positive by IEA. Of sera drawn earlier than 21 days from onset of primary genital HSV-2, antibodies to HSV-2 were detected in 25% by WBA and 8% by IEA. In patients with culture-proven primary genital HSV-1 infection, WBA detected antibodies to HSV-1 proteins in 16 of 17 (94%) serum samples drawn at least 21 days after onset of primary genital HSV-1 infection, compared with 9 of 17 (53%) serum samples tested for gG-1 by IEA. Both WBA and IEA are accurate and sensitive tests for HSV-2 antibody in patients convalescing from a first episode or having symptomatic or asymptomatic recurrent genital herpes. WBA was more sensitive than IEA in detecting seroconversion following primary HSV-1 genital herpes, although both assays may miss persons undergoing early seroconversion to HSV-2.

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• Liljeqvist, J.-A., Svennerholm, B., Bergström, T. (2000). Conservation of Type-Specific B-Cell Epitopes of Glycoprotein G in Clinical Herpes Simplex Virus Type 2 Isolates. J. Clin. Microbiol. 38: 4517-4522 [Abstract] [Full Text]  
• Koelle, D. M., Reymond, S. N., Chen, H., Kwok, W. W., McClurkan, C., Gyaltsong, T., Petersdorf, E. W., Rotkis, W., Talley, A. R., Harrison, D. A. (2000). Tegument-Specific, Virus-Reactive CD4 T Cells Localize to the Cornea in Herpes Simplex Virus Interstitial Keratitis in Humans. J. Virol. 74: 10930-10938 [Abstract] [Full Text]  
• Siscovick, D. S., Schwartz, S. M., Corey, L., Grayston, J. T., Ashley, R., Wang, S.-P., Psaty, B. M., Tracy, R. P., Kuller, L. H., Kronmal, R. A. (2000). Chlamydia pneumoniae, Herpes Simplex Virus Type 1, and Cytomegalovirus and Incident Myocardial Infarction and Coronary Heart Disease Death in Older Adults : The Cardiovascular Health Study. Circulation 102: 2335-2340 [Abstract] [Full Text]  
• Van Doornum, G. J. J., Slomka, M. J., Buimer, M., Groen, J., Van den Hoek, J. A. R., Cairo, I., Vyse, A., Brown, D. W. G. (2000). Comparison of a Monoclonal Antibody-Blocking Enzyme-Linked Immunoassay and a Strip Immunoblot Assay for Identifying Type-Specific Herpes Simplex Virus Type 2 Serological Responses. CVI 7: 641-644 [Abstract] [Full Text]  
• Lowhagen, G-B, Tunback, P, Andersson, K, Bergstrom, T, Johannisson, G (2000). First episodes of genital herpes in a Swedish STD population: a study of epidemiology and transmission by the use of herpes simplex virus (HSV) typing and specific serology. Sex. Transm. Infect. 76: 179-182 [Abstract] [Full Text]  
• Vyse, A J, Gay, N J, Slomka, M J, Gopal, R, Gibbs, T, Morgan-Capner, P, Brown, D W (2000). The burden of infection with HSV-1 and HSV-2 in England and Wales: implications for the changing epidemiology of genital herpes. Sex. Transm. Infect. 76: 183-187 [Abstract] [Full Text]  
• Kwok, W. W., Liu, A. W., Novak, E. J., Gebe, J. A., Ettinger, R. A., Nepom, G. T., Reymond, S. N., Koelle, D. M. (2000). HLA-DQ Tetramers Identify Epitope-Specific T Cells in Peripheral Blood of Herpes Simplex Virus Type 2-Infected Individuals: Direct Detection of Immunodominant Antigen-Responsive Cells. J. Immunol. 164: 4244-4249 [Abstract] [Full Text]  
• Wald, A., Zeh, J., Selke, S., Warren, T., Ryncarz, A. J., Ashley, R., Krieger, J. N., Corey, L. (2000). Reactivation of Genital Herpes Simplex Virus Type 2 Infection in Asymptomatic Seropositive Persons. NEJM 342: 844-850 [Abstract] [Full Text]  
• Corey, L., Handsfield, H. H. (2000). Genital Herpes and Public Health: Addressing a Global Problem. JAMA 283: 791-794 [Abstract] [Full Text]  
• Liljeqvist, J.-A., Svennerholm, B., Bergström, T. (1999). Herpes Simplex Virus Type 2 Glycoprotein G-Negative Clinical Isolates Are Generated by Single Frameshift Mutations. J. Virol. 73: 9796-9802 [Abstract] [Full Text]  
• Langenberg, A. G.M., Corey, L., Ashley, R. L., Leong, W. P., Straus, S. E., The Chiron HSV Vaccine Study Group, (1999). A Prospective Study of New Infections with Herpes Simplex Virus Type 1 and Type 2. NEJM 341: 1432-1438 [Abstract] [Full Text]  
• Rekabdar, E., Tunback, P., Liljeqvist, J.-A., Bergstrom, T. (1999). Variability of the Glycoprotein G Gene in Clinical Isolates of Herpes Simplex Virus Type 1. CVI 6: 826-831 [Abstract] [Full Text]  
• Ashley, R. L., Wald, A. (1999). Genital Herpes: Review of the Epidemic and Potential Use of Type-Specific Serology. Clin. Microbiol. Rev. 12: 1-8 [Abstract] [Full Text]  
• Koelle, D. M., Frank, J. M., Johnson, M. L., Kwok, W. W. (1998). Recognition of Herpes Simplex Virus Type 2 Tegument Proteins by CD4 T Cells Infiltrating Human Genital Herpes Lesions. J. Virol. 72: 7476-7483 [Abstract] [Full Text]  
• Ashley, R., Wu, L, Pickering, J., Tu, M., Schnorenberg, L (1998). Premarket evaluation of a commercial glycoprotein G-based enzyme immunoassay for herpes simplex virus type-specific antibodies [In Process Citation]. J. Clin. Microbiol. 36: 294-295 [Abstract] [Full Text]  
• Brown, Z. A., Selke, S., Zeh, J., Kopelman, J., Maslow, A., Ashley, R. L., Watts, D. H., Berry, S., Herd, M., Corey, L. (1997). The Acquisition of Herpes Simplex Virus during Pregnancy. NEJM 337: 509-516 [Abstract] [Full Text]  
• Wald, A., Zeh, J., Selke, S., Ashley, R. L., Corey, L. (1995). Virologic Characteristics of Subclinical and Symptomatic Genital Herpes Infections. NEJM 333: 770-775 [Abstract] [Full Text]  
• Cowan, F M, Johnson, A M, Ashley, R, Corey, L, Mindel, A (1994). Antibody to herpes simplex virus type 2 as serological marker of sexual lifestyle in populations. BMJ 309: 1325-1329 [Abstract] [Full Text]