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J Clin Microbiol. 1988 June; 26(6): 1227-1228

Rapid identification of Branhamella catarrhalis with 4-methylumbelliferyl butyrate.

M Vaneechoutte, G Verschraegen, G Claeys and P Flamen

Department of Medical Microbiology, University Hospital, Ghent, Belgium.

ABSTRACT

Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5 min, while Neisseria spp. remained negative, even after 18 h of incubation.


J Clin Microbiol. 1988 June; 26(6): 1227-1228







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