This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lipson, S M Articles by Szabo, K Search for Related Content PubMed PubMed Citation Articles by Lipson, S M Articles by Szabo, K

Sensitivity of rhabdomyosarcoma and guinea pig embryo cell cultures to field isolates of difficult-to-cultivate group A coxsackieviruses.

Department of Pathology and Laboratories, Nassau County Medical Center, East Meadow, New York 11554.

ABSTRACT

Forty-two difficult-to-cultivate group A coxsackieviruses (i.e., group A types other than A7, A9, and A16), collected primarily from throat swab specimens of patients suffering from fever, pharyngitis, lymphadenopathy, and cough during the 1986 enterovirus season, were isolated in less than 24-h-old suckling mice. Thirty-six moribund mice were sacrificed and autopsied, and then their brains and back musculature were inoculated into rhabdomyosarcoma (RD), guinea pig embryo (GPE), rhesus monkey kidney (RhMk) and human carcinoma of the larynx (HEp-2) cell cultures. Twelve of the 36 suckling mice isolates were adapted to grow in RD and GPE cells after two passes and have been identified in RD cells by type-specific antisera as group A coxsackievirus types A2, A4, and A8. Three passes in RhMk or HEp-2 cell cultures were insufficient to affect a discernible cytopathic effect. Coxsackievirus types A1, A19, and A22, unable to grow in any of the four cell cultures tested, were identified by virus neutralization in suckling mice. These data denote the efficacy of suckling mice for the isolation of difficult-to-cultivate group A coxsackieviruses.

This article has been cited by other articles:

• Witso, E., Palacios, G., Cinek, O., Stene, L. C., Grinde, B., Janowitz, D., Lipkin, W. I., Ronningen, K. S. (2006). High Prevalence of Human Enterovirus A Infections in Natural Circulation of Human Enteroviruses. J. Clin. Microbiol. 44: 4095-4100 [Abstract] [Full Text]  
• Espy, M. J., Uhl, J. R., Sloan, L. M., Buckwalter, S. P., Jones, M. F., Vetter, E. A., Yao, J. D. C., Wengenack, N. L., Rosenblatt, J. E., Cockerill, F. R. III, Smith, T. F. (2006). Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing. Clin. Microbiol. Rev. 19: 165-256 [Abstract] [Full Text]  
• Beld, M., Minnaar, R., Weel, J., Sol, C., Damen, M., van der Avoort, H., Wertheim-van Dillen, P., van Breda, A., Boom, R. (2004). Highly Sensitive Assay for Detection of Enterovirus in Clinical Specimens by Reverse Transcription-PCR with an Armored RNA Internal Control. J. Clin. Microbiol. 42: 3059-3064 [Abstract] [Full Text]  
• Nigrovic, L. E., Chiang, V. W. (2000). Cost Analysis of Enteroviral Polymerase Chain Reaction in Infants With Fever and Cerebrospinal Fluid Pleocytosis. Arch Pediatr Adolesc Med 154: 817-821 [Abstract] [Full Text]  
• Ramers, C., Billman, G., Hartin, M., Ho, S., Sawyer, M. H. (2000). Impact of a Diagnostic Cerebrospinal Fluid Enterovirus Polymerase Chain Reaction Test on Patient Management. JAMA 283: 2680-2685 [Abstract] [Full Text]  
• Muir, P., Kammerer, U., Korn, K., Mulders, M. N., Poyry, T., Weissbrich, B., Kandolf, R., Cleator, G. M., van Loon, A. M. (1998). Molecular Typing of Enteroviruses: Current Status and Future Requirements. Clin. Microbiol. Rev. 11: 202-227 [Abstract] [Full Text]