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Rapid and sensitive viral culture method for human immunodeficiency virus type 1.

Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455.

ABSTRACT

Peripheral blood mononuclear cells from 142 consecutive patients with antibodies to human immunodeficiency virus type 1 (HIV-1) were cultured for HIV-1. All 72 patients with symptoms of HIV-1 infection were culture positive, as were 69 of 70 asymptomatic patients. Of the 142 patients, 132 (93%) were culture positive within 10 days after initiation of the culture.

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