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J Clin Microbiol. 1988 August; 26(8): 1555-1560

Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II.

Center for Biotechnology, F82 Huddinge University Hospital, Sweden.

ABSTRACT

The gene for Escherichia coli heat-stable enterotoxin type II (STII) was fused to the genes for protein A from Staphylococcus aureus and beta-galactosidase in two different expression systems. Antibodies raised in rabbits against the protein A-STII fusion protein recognized the beta-galactosidase-STII fusion protein. The latter fusion protein was used as the immobilized antigen in an enzyme-linked immunosorbent assay (ELISA) for detection of STII. The correlation between the results of the ELISA and the intestinal loop test in piglets was 95%, suggesting that the ELISA can be used to reliably detect STII.