This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Langenberg, A Articles by Corey, L Search for Related Content PubMed PubMed Citation Articles by Langenberg, A Articles by Corey, L

Comparison of diploid fibroblast and rabbit kidney tissue cultures and a diploid fibroblast microtiter plate system for the isolation of herpes simplex virus.

Department of Laboratory Medicine, University of Washington, Seattle 98105.

ABSTRACT

We evaluated the relative sensitivities of two cell systems (rabbit kidney [RK] and human diploid fibroblast [DF; human embryonic tonsil]) in standard tube cultures versus DF cells in a 48-well microtiter plate system for the detection of both symptomatic and asymptomatic herpes simplex virus (HSV) infection. At least one system isolated HSV in 111 of 809 specimens (13.7%). HSV was isolated in RK tube cultures from 110 specimens (99%), in DF tube cultures from 91 specimens (82%), and in DF microtiter plates from 95 specimens (86%). The frequency of HSV isolation varied with the anatomic site and the presence or absence of a herpetic lesion. The sensitivities of the three culture systems remained similar whether the specimens were obtained from lesions or whether the specimens were taken to determine if asymptomatic excretion of HSV was present. While RK tube cultures were more sensitive than DF tube cultures, the DF microtiter plate system was as sensitive as DF tube cultures and its use is supported as a cheaper and less labor-intensive method for the detection of HSV.

This article has been cited by other articles:

• Wald, A, Ericsson, M, Krantz, E, Selke, S, Corey, L (2004). Oral shedding of herpes simplex virus type 2. Sex. Transm. Infect. 80: 272-276 [Abstract] [Full Text]  
• Posavad, C. M., Wald, A., Hosken, N., Huang, M. L., Koelle, D. M., Ashley, R. L., Corey, L. (2003). T Cell Immunity to Herpes Simplex Viruses in Seronegative Subjects: Silent Infection or Acquired Immunity?. J. Immunol. 170: 4380-4388 [Abstract] [Full Text]  
• Brown, Z. A., Wald, A., Morrow, R. A., Selke, S., Zeh, J., Corey, L. (2003). Effect of Serologic Status and Cesarean Delivery on Transmission Rates of Herpes Simplex Virus From Mother to Infant. JAMA 289: 203-209 [Abstract] [Full Text]  
• Wald, A., Langenberg, A. G. M., Link, K., Izu, A. E., Ashley, R., Warren, T., Tyring, S., Douglas, J. M. Jr, Corey, L. (2001). Effect of Condoms on Reducing the Transmission of Herpes Simplex Virus Type 2 From Men to Women. JAMA 285: 3100-3106 [Abstract] [Full Text]  
• Wald, A., Zeh, J., Selke, S., Warren, T., Ryncarz, A. J., Ashley, R., Krieger, J. N., Corey, L. (2000). Reactivation of Genital Herpes Simplex Virus Type 2 Infection in Asymptomatic Seropositive Persons. NEJM 342: 844-850 [Abstract] [Full Text]  
• Eis-Hübinger, A. M., Däumer, M., Matz, B., Schneweis, K. E. (1999). Evaluation of Three Glycoprotein G2-Based Enzyme Immunoassays for Detection of Antibodies to Herpes Simplex Virus Type 2 in Human Sera. J. Clin. Microbiol. 37: 1242-1246 [Abstract] [Full Text]  
• Schacker, T., Ryncarz, A. J., Goddard, J., Diem, K., Shaughnessy, M., Corey, L. (1998). Frequent Recovery of HIV-1 From Genital Herpes Simplex Virus Lesions in HIV-1-Infected Men. JAMA 280: 61-66 [Abstract] [Full Text]  
• Schacker, T., Hu, H.-l., Koelle, D. M., Zeh, J., Saltzman, R., Boon, R., Shaughnessy, M., Barnum, G., Corey, L. (1998). Famciclovir for the Suppression of Symptomatic and Asymptomatic Herpes Simplex Virus Reactivation in HIV-Infected Persons: A Double-Blind, Placebo-Controlled Trial. ANN INTERN MED 128: 21-28 [Abstract] [Full Text]