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J Clin Microbiol. 1989 December; 27(12): 2730-2735

Cloned DNA probes identify Anaplasma ovis in goats and reveal a high prevalence of infection.

Immunology Section, Veterinary Research Laboratory Kabete, Kenya.

ABSTRACT

Anaplasma organisms are observed in erythrocytes from goats with anemia and weight loss in Kenya. Three anaplasmas have been isolated in nature, Anaplasma ovis, Anaplasma marginale, and Anaplasma centrale. The two recognized species, A. ovis and A. marginale, are known to infect goats. Since only A. ovis causes clinical disease in goats, the Anaplasma species in goats in Kenya were identified. To detect A. ovis, a 9.6-kilobase-pair section of genomic DNA was cloned into pBR322 (pAO12A) and was used in conjunction with an A. marginale DNA probe previously derived from a gene coding for a 105,000-molecular-weight surface protein (Am105L) of A. marginale. In Southern blots, pAO12A DNA hybridized to several at least partially homologous sequences that were present in A. ovis and A. marginale genomic DNAs. The pAO12A DNA did not hybridize to Babesia bovis genomic or goat leukocyte DNA. The Anaplasma species that infected goats was identified as A. ovis by (i) DNA hybridization with pAO12A, (ii) hybridization of the A. marginale DNA probe to A. centrale and A. marginale genomic DNAs and lack of hybridization to A. ovis genomic DNA from an isolate obtained in Idaho and Anaplasma DNA from infected goats in Kenya, (iii) the intraerythrocytic location of Anaplasma organisms in infected goat blood, and (iv) the host specificity of the Anaplasma organisms for goats but not for cattle. Also, by using the two Anaplasma DNA probes, the prevalence of A. ovis in goats from seven locations in Kenya was found to range from 22 to 87%. The pAO12A DNA probe detected a 0.0035% A. ovis parasitemia in infected blood, an improved sensitivity which is suitable for use in surveillance and epidemiological studies.