This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by LeFebvre, R B Articles by Perng, G C Search for Related Content PubMed PubMed Citation Articles by LeFebvre, R B Articles by Perng, G C

 Previous Article  |  Next Article 

J Clin Microbiol. 1989 March; 27(3): 389-393

Genetic and antigenic characterization of Borrelia coriaceae, putative agent of epizootic bovine abortion.

Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.

ABSTRACT

Borrelia coriaceae was characterized genetically and antigenically by utilizing the following techniques: restriction endonuclease analysis, Southern blotting and genomic hybridization, pulsed-field electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting. The B. coriaceae genome revealed unique and characteristic banding patterns both by agarose gel electrophoresis and by hybridization when compared with several Borrelia burgdorferi isolates. Pulsed-field gel electrophoresis demonstrated several linear plasmids ranging from 65 to 30 kilobase pairs. Cross-reaction with B. burgdorferi antigens ranging from 21 to 26 kilodaltons were demonstrated by immunoblotting with rabbit anti-B. coriaceae antiserum. However, most B. coriaceae antigens were quite distinct when compared with B. burgdorferi and Leptospira interrogans antigens.

This article has been cited by other articles:

• Hendson, M., Lane, R. S. (2000). Genetic Characteristics of Borrelia coriaceae Isolates from the Soft Tick Ornithodoros coriaceus (Acari: Argasidae). J. Clin. Microbiol. 38: 2678-2682 [Abstract] [Full Text]