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J Clin Microbiol. 1989 August; 27(8): 1854-1858

Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi.

K E Hechemy, H L Harris, J A Wethers, R W Stevens, B R Stock, A A Reilly and J L Benach

Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.

ABSTRACT

Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.

J Clin Microbiol. 1989 August; 27(8): 1854-1858






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1989 by the American Society for Microbiology. All rights reserved.