This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Loeffelholz, M J Articles by Scholl, D R Search for Related Content PubMed PubMed Citation Articles by Loeffelholz, M J Articles by Scholl, D R

 Previous Article  |  Next Article 

J Clin Microbiol. 1989 August; 27(8): 1880-1881

Method for improved extraction of DNA from Nocardia asteroides.

Diagnostic Hybrids, Inc., Athens, Ohio 45701.

ABSTRACT

In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60 degrees C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.

This article has been cited by other articles:

• Conville, P. S., Brown, J. M., Steigerwalt, A. G., Brown-Elliott, B. A., Witebsky, F. G. (2008). Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., Human Pathogens and Members of the "Nocardia transvalensis Complex". J. Clin. Microbiol. 46: 1178-1184 [Abstract] [Full Text]  
• Xu, J.-L., He, J., Wang, Z.-C., Wang, K., Li, W.-J., Tang, S.-K., Li, S.-P. (2007). Rhodococcus qingshengii sp. nov., a carbendazim-degrading bacterium. Int. J. Syst. Evol. Microbiol. 57: 2754-2757 [Abstract] [Full Text]  
• Conville, P. S., Witebsky, F. G. (2007). Analysis of Multiple Differing Copies of the 16S rRNA Gene in Five Clinical Isolates and Three Type Strains of Nocardia Species and Implications for Species Assignment. J. Clin. Microbiol. 45: 1146-1151 [Abstract] [Full Text]  
• Greenberg, D. E., Porcella, S. F., Stock, F., Wong, A., Conville, P. S., Murray, P. R., Holland, S. M., Zelazny, A. M. (2006). Granulibacter bethesdensis gen. nov., sp. nov., a distinctive pathogenic acetic acid bacterium in the family Acetobacteraceae.. Int. J. Syst. Evol. Microbiol. 56: 2609-2616 [Abstract] [Full Text]  
• Butler, W. R., Floyd, M. M., Brown, J. M., Toney, S. R., Daneshvar, M. I., Cooksey, R. C., Carr, J., Steigerwalt, A. G., Charles, N. (2005). Novel mycolic acid-containing bacteria in the family Segniliparaceae fam. nov., including the genus Segniliparus gen. nov., with descriptions of Segniliparus rotundus sp. nov. and Segniliparus rugosus sp. nov.. Int. J. Syst. Evol. Microbiol. 55: 1615-1624 [Abstract] [Full Text]  
• Conville, P. S., Witebsky, F. G. (2005). Multiple Copies of the 16S rRNA Gene in Nocardia nova Isolates and Implications for Sequence-Based Identification Procedures. J. Clin. Microbiol. 43: 2881-2885 [Abstract] [Full Text]  
• Schinsky, M. F., Morey, R. E., Steigerwalt, A. G., Douglas, M. P., Wilson, R. W., Floyd, M. M., Butler, W. R., Daneshvar, M. I., Brown-Elliott, B. A., Wallace, R. J. Jr, McNeil, M. M., Brenner, D. J., Brown, J. M. (2004). Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates. Int. J. Syst. Evol. Microbiol. 54: 1653-1667 [Abstract] [Full Text]  
• Jones, A. L., Brown, J. M., Mishra, V., Perry, J. D., Steigerwalt, A. G., Goodfellow, M. (2004). Rhodococcus gordoniae sp. nov., an actinomycete isolated from clinical material and phenol-contaminated soil. Int. J. Syst. Evol. Microbiol. 54: 407-411 [Abstract] [Full Text]  
• Conville, P. S., Brown, J. M., Steigerwalt, A. G., Lee, J. W., Byrer, D. E., Anderson, V. L., Dorman, S. E., Holland, S. M., Cahill, B., Carroll, K. C., Witebsky, F. G. (2003). Nocardia veterana as a Pathogen in North American Patients. J. Clin. Microbiol. 41: 2560-2568 [Abstract] [Full Text]