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J Clin Microbiol. 1990 January; 28(1): 159-162

Rapid quantitation of DNA spot hybridization by flatbed scintillation counting.

T Hyypiä, E Auvinen, S Kovanen and T H Ståhlberg

Department of Virology, University of Turku, Finland.

ABSTRACT

The flatbed scintillation counting system (Betaplate) was used for quantitative measurement of the radioactive hybridization signal in detection of adenovirus and papillomavirus DNA in clinical specimens. In this method, 96 samples on a nylon membrane can be handled as a single entity throughout the hybridization and counting procedure. The technique is sensitive, rapid, and convenient in routine use when compared with conventionally applied methods for the numerical analysis of hybridization results. The assay principle allows simultaneous testing of large numbers of specimens.

J Clin Microbiol. 1990 January; 28(1): 159-162






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