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J Clin Microbiol. 1990 November; 28(11): 2415-2417

Association of production of cell-detaching factor with the clinical presentation of Trichomonas vaginalis.

Department of Medicine, University of Ottawa, Ontario, Canada.

ABSTRACT

Recent work has shown that Trichomonas vaginalis produces a cell-detaching factor (CDF) that causes detachment of monolayer cells in vitro. To study the role of CDF as a pathogenic marker of disease, we studied the production of CDF in 12 clinical isolates of T. vaginalis. These isolates were also utilized in the mouse subcutaneous assay of Honigberg, which is the standard for pathogenicity of T. vaginalis. The isolates were divided into three groups based on clinical presentation (asymptomatic [n = 4], moderate [n = 4], and severe symptoms [n = 4]). CDF was assessed by harvesting the supernatant from the growth of T. vaginalis in cell culture and filtering the supernatant through a 0.45-microns-pore-size filter. The filtrate was applied in a microtiter cytotoxicity assay. The mouse subcutaneous assay did not significantly differentiate among the isolates. However, CDF was strongly associated with clinical presentation by two-way, repeated-measure analysis of variance (P = 0.025). Thus, CDF appears to correlate with clinical presentation and may be an important virulence marker in T. vaginalis pathogenesis.

This article has been cited by other articles:

• Petrin, D., Delgaty, K., Bhatt, R., Garber, G. (1998). Clinical and Microbiological Aspects of Trichomonas vaginalis. Clin. Microbiol. Rev. 11: 300-317 [Abstract] [Full Text]