This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mogollon, J D Articles by Cleary, P P Search for Related Content PubMed PubMed Citation Articles by Mogollon, J D Articles by Cleary, P P

 Previous Article  |  Next Article 

J Clin Microbiol. 1990 November; 28(11): 2462-2466

Characterization of prototype and clinically defined strains of Streptococcus suis by genomic fingerprinting.

Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, Minneapolis 55108.

ABSTRACT

A collection of Streptococcus suis strains from animal and human infections was examined for DNA-banding patterns after restriction endonuclease digestion and agarose gel electrophoresis. The endonuclease HaeIII produced the most discriminating restriction profiles among 23 serotypes studied. DNA from serotypes 9, 11, 12, and 16 was resistant to HaeIII cleavage. DNA from serotypes 9 through 16 was cleaved with HindIII and showed substantial genomic differences. We also examined 106 epidemiologically unrelated strains isolated from cases of pig meningitis or pneumonia and 5 strains isolated from cases of human meningitis in order to compare genomic fingerprinting and serotyping as epidemiological tools. Heterogeneity was found among fingerprints of serologically identical isolates, indicating genetic diversity within some serotypes. DNA fingerprints of some serotype 2 strains from different sources appeared identical, suggesting a clonal relationship among strains of this serotype. The data suggest that this technique represents an important tool for examining the natural history of disease caused by S. suis.

This article has been cited by other articles:

• King, S. J., Leigh, J. A., Heath, P. J., Luque, I., Tarradas, C., Dowson, C. G., Whatmore, A. M. (2002). Development of a Multilocus Sequence Typing Scheme for the Pig Pathogen Streptococcus suis: Identification of Virulent Clones and Potential Capsular Serotype Exchange. J. Clin. Microbiol. 40: 3671-3680 [Abstract] [Full Text]  
• Chatellier, S., Gottschalk, M., Higgins, R., Brousseau, R., Harel, J. (1999). Relatedness of Streptococcus suis Serotype 2 Isolates from Different Geographic Origins as Evaluated by Molecular Fingerprinting and Phenotyping. J. Clin. Microbiol. 37: 362-366 [Abstract] [Full Text]  
• Rasmussen, S. R., Aarestrup, F. M., Jensen, N. E., Jorsal, S. E. (1999). Associations of Streptococcus suis Serotype 2 Ribotype Profiles with Clinical Disease and Antimicrobial Resistance. J. Clin. Microbiol. 37: 404-408 [Abstract] [Full Text]  
• Staats, J., Plattner, B., Nietfeld, J, Dritz, S, Chengappa, M. (1998). Use of ribotyping and hemolysin activity to identify highly virulent Streptococcus suis type 2 isolates [In Process Citation]. J. Clin. Microbiol. 36: 15-19 [Abstract] [Full Text]  
• Weinstein, M. R., Litt, M., Kertesz, D. A., Wyper, P., Rose, D., Coulter, M., McGeer, A., Facklam, R., Ostach, C., Willey, B. M., Borczyk, A., Low, D. E., The S. iniae Study Group, (1997). Invasive Infections Due to a Fish Pathogen, Streptococcus iniae. NEJM 337: 589-594 [Abstract] [Full Text]