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J Clin Microbiol. 1990 April; 28(4): 798-800

Simple assay of calcium dependency for virulent plasmid-bearing clones of Yersinia enterocolitica.

Eastern Regional Research Center, U.S. Department of Agriculture, Philadelphia, Pennsylvania 19118.

ABSTRACT

A simplified procedure to detect the calcium dependency of virulent plasmid-bearing strains of Yersinia enterocolitica was developed. A low-calcium, agarose-based medium of brain heat infusion with added magnesium effectively differentiated plasmid-bearing and plasmidless isolates. Further, the expression of calcium dependency in plasmid-bearing strains of Y. enterocolitica as measured by the average colony diameter was proportional to the calcium concentration of the assay.

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