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J Clin Microbiol. 1990 July; 28(7): 1525-1529

Laboratory identification of adenoviruses associated with gastroenteritis in Canada from 1983 to 1986.

Department of Microbiology, University of Toronto, Ontario, Canada.

ABSTRACT

A retrospective analysis of adenovirus serotypes associated with gastroenteritis involved the examination of 143 stool specimens collected between 1983 and 1986 from symptomatic patients whose stools were positive for adenovirus by electron microscopy. The virus isolates obtained from 140 of the specimens were typed according to the SmaI cleavage pattern of the viral DNA and by neutralization with specific antisera. The predominant types were adenovirus type 31 (Ad31) (18%), Ad40 (16.9%), and Ad41 (38%), which together accounted for more than 70% of the isolates. The remaining virus isolates were typed as Ad1, 2, 3, 5, 7, and 12. DNA restriction analysis proved to be better than serum neutralization for identification of the enteric adenovirus serotypes in stool specimens. HindIII cleavage identified four Ad41 variants, none of which had a HindIII restriction pattern identical to that of the prototype strain Tak. Over the time period of the study, the incidence of Ad40 showed an overall decrease accompanied by an increased incidence of Ad41, while the incidence of Ad31 was relatively stable.

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