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J Clin Microbiol. 1990 August; 28(8): 1701-1703

Processing and microfiltration of mosquitoes for malaria antigen detection in a rapid dot immunobinding assay.

Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814.

ABSTRACT

Data on a technique for the detection of antigen from arthropod vectors in a dot immunobinding assay are presented. In this system, antigen present in the vector was first solubilized in sodium dodecyl sulfate. The homogenate from this process was microfiltered through a two-membrane sandwich; target antigen molecules passed through the first membrane and were immobilized on the second one. The first membrane was nonbinding and served to impinge debris. The second membrane was a high-protein-binding-capacity hydrophobic polyvinylidene difluoride membrane. High signal-to-noise ratios were produced by this method, which is readily adaptable for field use. This assay was used for malaria sporozoites, but it can serve as a general technique that is applicable to other arthropod vectors and etiologic agents.