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J Clin Microbiol. 1990 August; 28(8): 1851-1853

Presumptive identification of Clostridium difficile by detection of p-cresol in prepared peptone yeast glucose broth supplemented with p-hydroxyphenylacetic acid.

Department of Laboratory Medicine, Calgary General Hospital, Alberta, Canada.

ABSTRACT

Prereduced, anaerobically sterilized peptone yeast glucose broth was supplemented with p-hydroxyphenylacetic acid and used for the presumptive identification of Clostridium difficile. Two hundred eighty-two organisms were grown in this medium for 18 h and tested for p-cresol production by gas-liquid chromatography. All 49 stock and reference strains of C. difficile and 19 organisms confirmed as C. difficile produced p-cresol. p-Cresol was not produced by 53 negative control or 161 test organisms. The system was convenient and effective.

This article has been cited by other articles:

• Dawson, L. F., Stabler, R. A., Wren, B. W. (2008). Assessing the role of p-cresol tolerance in Clostridium difficile. J Med Microbiol 57: 745-749 [Abstract] [Full Text]