This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Freney, J Articles by Fleurette, J Search for Related Content PubMed PubMed Citation Articles by Freney, J Articles by Fleurette, J

Evaluation of API Coryne in comparison with conventional methods for identifying coryneform bacteria.

Laboratoire de Bactériologie, Faculté de Pharmacie, Lyon, France.

ABSTRACT

A study was performed to evaluate a new manual miniaturized system, API Coryne (API-bioMérieux, Inc., La Balme les Grottes, France), in which conventional biochemical methods were used to identify 240 isolates of coryneform and related bacteria. A total of 40% of the isolates were excluded from the study because they could not be identified by conventional methods. Identifications of the 240 isolates obtained with API Coryne showed a 97.6% concordance with conventional methods (79% after 24 h of incubation, 21% after 48 h of incubation): 158 (65.8%) isolates were identified with no further testing, and extra testing was required for 76 (31.8%) isolates. In three (1.2%) cases, the organisms did not correspond to any key in the code book and could not be identified by the computer service of the manufacturer. Only three (1.2%) strains were misidentified. The system was shown to be reliable and rapid when compared with standard identification methods.

This article has been cited by other articles:

• Adderson, E. E., Boudreaux, J. W., Cummings, J. R., Pounds, S., Wilson, D. A., Procop, G. W., Hayden, R. T. (2008). Identification of Clinical Coryneform Bacterial Isolates: Comparison of Biochemical Methods and Sequence Analysis of 16S rRNA and rpoB Genes. J. Clin. Microbiol. 46: 921-927 [Abstract] [Full Text]  
• Renom, F., Garau, M., Rubi, M., Ramis, F., Galmes, A., Soriano, J. B. (2007). Nosocomial Outbreak of Corynebacterium striatum Infection in Patients with Chronic Obstructive Pulmonary Disease. J. Clin. Microbiol. 45: 2064-2067 [Abstract] [Full Text]  
• Tang, Y.-W., Von Graevenitz, A., Waddington, M. G., Hopkins, M. K., Smith, D. H., Li, H., Kolbert, C. P., Montgomery, S. O., Persing, D. H. (2000). Identification of Coryneform Bacterial Isolates by Ribosomal DNA Sequence Analysis. J. Clin. Microbiol. 38: 1676-1678 [Abstract] [Full Text]  
• Renaud, F. N. R., Dutaur, M., Daoud, S., Aubel, D., Riegel, P., Monget, D., Freney, J. (1998). Differentiation of Corynebacterium amycolatum, C. minutissimum, and C. striatum by Carbon Substrate Assimilation Tests. J. Clin. Microbiol. 36: 3698-3702 [Abstract] [Full Text]  
• Hudspeth, M. K., Gerardo, S. H., Citron, D. M., Goldstein, E. J. C. (1998). Evaluation of the RapID CB Plus System for Identification of Corynebacterium Species and Other Gram-Positive Rods. J. Clin. Microbiol. 36: 543-547 [Abstract] [Full Text]