This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oswald, E Articles by Pohl, P Search for Related Content PubMed PubMed Citation Articles by Oswald, E Articles by Pohl, P

Virulence factors associated with cytotoxic necrotizing factor type two in bovine diarrheic and septicemic strains of Escherichia coli.

Laboratoire de Pathologie Infectieuse et d'Immunologie, Institut National de la Recherche Agronomique, Nouzilly, France.

ABSTRACT

Forty-three bovine isolates of Escherichia coli producing a second type of cytotoxic necrotizing factor (CNF2) and three K-12 strains carrying different Vir plasmids coding for CNF2 were tested for the presence of several virulence factors. Most of the strains were serum resistant (79%), produced an aerobactin (70%), and adhered to calf villi (53%); some of them produced a colicin (32%) and a hemolysin (9%). These strains were also tested by a colony hybridization assay with gene probes for six toxins (classical heat-stable [STaP and STb] and heat-labile [LT-I and LT-IIa] enterotoxins and Shiga-like toxins [SLT-I and SLT-II]) and five adhesion factors (K99, K88, 987P, F17, and F41). Only two gene probes, LT-IIa (9%) and F17A (53%), hybridized with the CNF2 strains. However, antibodies raised against F17 fimbriae did not agglutinate the strains hybridizing with the F17A probe. In contrast, all except one of these strains adhered to calf villi. Interestingly, these two properties, F17A positivity and adherence to calf villi, were the only ones expressed by the K-12 strains carrying different Vir plasmids. In conclusion, this study confirmed that CNF2-producing strains are unrelated to previously described toxigenic E. coli strains and also demonstrated that in half of the strains the production of CNF2 was associated with an adhesion factor genetically related to, but different from, F17, which is more than likely encoded by Vir plasmids.

This article has been cited by other articles:

• Toth, I., Herault, F., Beutin, L., Oswald, E. (2003). Production of Cytolethal Distending Toxins by Pathogenic Escherichia coli Strains Isolated from Human and Animal Sources: Establishment of the Existence of a New cdt Variant (Type IV). J. Clin. Microbiol. 41: 4285-4291 [Abstract] [Full Text]  
• Van Bost, S., Jacquemin, E., Oswald, E., Mainil, J. (2003). Multiplex PCRs for Identification of Necrotoxigenic Escherichia coli. J. Clin. Microbiol. 41: 4480-4482 [Abstract] [Full Text]  
• Lockman, H. A., Gillespie, R. A., Baker, B. D., Shakhnovich, E. (2002). Yersinia pseudotuberculosis Produces a Cytotoxic Necrotizing Factor. Infect. Immun. 70: 2708-2714 [Abstract] [Full Text]  
• Hilali, F., Ruimy, R., Saulnier, P., Barnabe, C., Lebouguenec, C., Tibayrenc, M., Andremont, A. (2000). Prevalence of Virulence Genes and Clonality in Escherichia coli Strains That Cause Bacteremia in Cancer Patients. Infect. Immun. 68: 3983-3989 [Abstract] [Full Text]  
• Island, M. D., Cui, X., Foxman, B., Marrs, C. F., Stamm, W. E., Stapleton, A. E., Warren, J. W. (1998). Cytotoxicity of Hemolytic, Cytotoxic Necrotizing Factor 1-Positive and -Negative Escherichia coli to Human T24 Bladder Cells. Infect. Immun. 66: 3384-3389 [Abstract] [Full Text]  
• Elliott, S. J., Srinivas, S., Albert, M. J., Alam, K., Robins-Browne, R. M., Gunzburg, S. T., Mee, B. J., Chang, B. J. (1998). Characterization of the Roles of Hemolysin and Other Toxins in Enteropathy Caused by Alpha-Hemolytic Escherichia coli Linked to Human Diarrhea. Infect. Immun. 66: 2040-2051 [Abstract] [Full Text]