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Quantitative microculture system with standardized inocula for strain typing, susceptibility testing, and other physiologic measurements with Candida albicans and other yeasts.

Department of Bacteriology and Mycology, Janssen Research Foundation, Beerse, Belgium.

ABSTRACT

A method for physiologic testing of Candida albicans and other yeasts is described in which inocula with reproducible cell concentrations are prepared from cultures in a glucose-limited medium and growth turbidities of test cultures in microplates are expressed quantitatively as a percentage of control growth. The method gave good within-plate and run-to-run reproducibilities and was applicable both to strain phenotype discrimination in C. albicans and to susceptibility tests of yeasts with azole antifungal agents.

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