This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carnahan, A M Articles by Joseph, S W Search for Related Content PubMed PubMed Citation Articles by Carnahan, A M Articles by Joseph, S W

Aerokey II: a flexible key for identifying clinical Aeromonas species.

Department of Microbiology, University of Maryland, College Park 20742.

ABSTRACT

A small subset (n = 18) of highly discriminatory tests was derived from the feature frequency of 50 tests used in the study of 167 predominantly clinical Aeromonas strains. Seven of these eighteen tests were used to construct a flexible, dichotomous key, Aerokey II, for identifying clinical aerontonads: esculin hydrolysis, gas from glucose, acid from arabinose, indole production, acid from sucrose, Voges-Proskauer reaction, and resistance to cephalothin (30 micrograms). This schema was initially evaluated in a single-blind trial of 60 well-characterized clinical Aeromonas hydrophila (n = 21), A. caviae (n = 19), and A. veronii bv. sobria (n = 20) strains from an independent laboratory. Of the 60 strains tested, 58 (97%) were accurately identified to the species level. Aerokey II was further evaluated with 18 additional American Type Culture Collection and reference strains representing the more recently proposed taxa A. veronii bv. veronii, A. schubertii, A. jandaei, and A. trota and accurately identified all of these strains.

This article has been cited by other articles:

• Sinha, S., Shimada, T., Ramamurthy, T., Bhattacharya, S. K., Yamasaki, S., Takeda, Y., Nair, G. B. (2004). Prevalence, serotype distribution, antibiotic susceptibility and genetic profiles of mesophilic Aeromonas species isolated from hospitalized diarrhoeal cases in Kolkata, India. J Med Microbiol 53: 527-534 [Abstract] [Full Text]  
• Soler, L., Marco, F., Vila, J., Chacon, M. R., Guarro, J., Figueras, M. J. (2003). Evaluation of Two Miniaturized Systems, MicroScan W/A and BBL Crystal E/NF, for Identification of Clinical Isolates of Aeromonas spp.. J. Clin. Microbiol. 41: 5732-5734 [Abstract] [Full Text]  
• Esteve, C., Valera, L., Gutierrez, C., Ventosa, A. (2003). Taxonomic study of sucrose-positive Aeromonas jandaei-like isolates from faeces, water and eels: emendation of A. jandaei Carnahan et al. 1992. Int. J. Syst. Evol. Microbiol. 53: 1411-1419 [Abstract] [Full Text]  
• Abbott, S. L., Cheung, W. K. W., Janda, J. M. (2003). The Genus Aeromonas: Biochemical Characteristics, Atypical Reactions, and Phenotypic Identification Schemes. J. Clin. Microbiol. 41: 2348-2357 [Abstract] [Full Text]  
• Villari, P., Crispino, M., Montuori, P., Boccia, S. (2003). Molecular Typing of Aeromonas Isolates in Natural Mineral Waters. Appl. Environ. Microbiol. 69: 697-701 [Abstract] [Full Text]  
• O'Hara, C. M., Miller, J. M. (2002). Ability of the MicroScan Rapid Gram-Negative ID Type 3 Panel To Identify Nonenteric Glucose-Fermenting and Nonfermenting Gram-Negative Bacilli. J. Clin. Microbiol. 40: 3750-3752 [Abstract] [Full Text]  
• Rahman, M., Colque-Navarro, P., Kuhn, I., Huys, G., Swings, J., Mollby, R. (2002). Identification and Characterization of Pathogenic Aeromonas veronii Biovar Sobria Associated with Epizootic Ulcerative Syndrome in Fish in Bangladesh. Appl. Environ. Microbiol. 68: 650-655 [Abstract] [Full Text]  
• Kirov, S. M., Tassell, B. C., Semmler, A. B. T., O'Donovan, L. A., Rabaan, A. A., Shaw, J. G. (2002). Lateral Flagella and Swarming Motility in Aeromonas Species. J. Bacteriol. 184: 547-555 [Abstract] [Full Text]  
• KRZYMINSKA, S., MOKRACKA, J., LAGANOWSKA, M., WLODARCZAK, K., GUSZCZYNSKA, E. B., LISZKOWSKA, J., POPKOWSKA, E., LIMA, I., LEMANSKA, I., WENDT, M. (2001). Enhancement of the virulence of Aeromonas caviae diarrhoeal strains by serial passages in mice. J Med Microbiol 50: 303-312 [Abstract] [Full Text]  
• Kirov, S. M., O'Donovan, L. A., Sanderson, K. (1999). Functional Characterization of Type IV Pili Expressed on Diarrhea-Associated Isolates of Aeromonas species. Infect. Immun. 67: 5447-5454 [Abstract] [Full Text]  
• Khan, A. A., Kim, E., Cerniglia, C. E. (1998). Molecular Cloning, Nucleotide Sequence, and Expression in Escherichia coli of a Hemolytic Toxin (Aerolysin) Gene from Aeromonas trota. Appl. Environ. Microbiol. 64: 2473-2478 [Abstract] [Full Text]