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J Clin Microbiol. 1991 February; 29(2): 355-358

Chemotypes of Mycobacterium malmoense based on glycolipid profiles.

M L Katila, E Brander, E Jantzen, R Huttunen and L Linkosalo

Department of Clinical Microbiology, Kuopio University Central Hospital, Finland.

ABSTRACT

Thin-layer chromatographic analysis of 72 Finnish clinical mycobacterial isolates presumptively identified as Mycobacterium malmoense revealed four major glycolipid profiles with two minor variations. An additional glycolipid profile was found in three British M. malmoense-like strains. No clear distinction between the strains could be made by means of gas chromatography of cellular fatty acids. The two M. malmoense-specific constituents, 2-methyleicosanoate and 2,4,6-trimethyltetracosanoate, were detected in all strains. The frequency of chemotypes other than that of the type strain was 8% among the Finnish isolates. This variation should be recognized when confirmative identification of mycobacteria is based on thin-layer chromatography of glycolipid extracts.


J Clin Microbiol. 1991 February; 29(2): 355-358




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