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J Clin Microbiol. 1991 February; 29(2): 367-372

Several groups among human herpesvirus 6 strains can be distinguished by Southern blotting and polymerase chain reaction.

J T Aubin, H Collandre, D Candotti, D Ingrand, C Rouzioux, M Burgard, S Richard, J M Huraux and H Agut

Laboratoire de Bactériologie-Virologie, C.E.R.V.I., Hôpital de la Pitié-Salpêtrière, Paris, France.

ABSTRACT

Eight human herpesvirus 6 (HHV-6) strains were studied by Southern blot and polymerase chain reaction. DNA from infected cells was digested by a panel of restriction enzymes and hybridized with cloned BamHI fragments corresponding to about 30% of the HHV-6 strain SIE genome. In parallel, this DNA was amplified by polymerase chain reaction using pairs of primers derived from the strain SIE nucleotide sequence. Subsequently, amplification products were analyzed by hybridization, digestion with restriction endonucleases, and partial nucleotide sequencing. Overall results indicated that all strains were closely related to one another. However, concordant differences in restriction patterns allowed at least two groups to be distinguished, typified by strains SIE and HST, respectively. Differences between the two groups were found to reflect a limited number of punctual changes in nucleotide sequences. These results strengthen the idea of a unique HHV-6 species with genetic polymorphism. In addition, this study provides useful markers for the diagnosis and molecular epidemiology of HHV-6 infections.

J Clin Microbiol. 1991 February; 29(2): 367-372




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Copyright © 1991 by the American Society for Microbiology. All rights reserved.