This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Andersen, A A Search for Related Content PubMed PubMed Citation Articles by Andersen, A A

Serotyping of Chlamydia psittaci isolates using serovar-specific monoclonal antibodies with the microimmunofluorescence test.

National Animal Disease Center, U.S. Department of Agriculture, Ames, Iowa 50010.

ABSTRACT

A panel of 10 serovar-specific monoclonal antibodies that could distinguish 10 distinct serovars of Chlamydia psittaci was prepared. The panel included one monoclonal antibody to each of the 10 serovars. Monoclonal antibodies were selected for their specificity in the indirect microimmunofluorescence test. Each of the monoclonal antibodies had a titer of 1:1,280 or higher to the homologous strain, with only two showing any cross-reactivity at a dilution of 1:10. Chlamydial antigen derived from organisms growing in tissue culture of one well of a 96-well multiwell dish was usually sufficient for the serotyping of an isolate. Infected yolk sac preparations were also suitable for serotyping. The panel of monoclonal antibodies was used to serotype 55 mammalian and avian strains. All except five of the strains were successfully serotyped; these five strains are presumed to represent at least two additional serovars. The use of a panel of monoclonal antibodies in the indirect microimmunofluorescence test provides a rapid and reliable method for serotyping new isolates. Monoclonal antibodies to new serovars can easily be added to the panel.

This article has been cited by other articles:

• Verminnen, K., Duquenne, B., De Keukeleire, D., Duim, B., Pannekoek, Y., Braeckman, L., Vanrompay, D. (2008). Evaluation of a Chlamydophila psittaci Infection Diagnostic Platform for Zoonotic Risk Assessment. J. Clin. Microbiol. 46: 281-285 [Abstract] [Full Text]  
• Harkinezhad, T., Verminnen, K., Van Droogenbroeck, C., Vanrompay, D. (2007). Chlamydophila psittaci genotype E/B transmission from African grey parrots to humans. J Med Microbiol 56: 1097-1100 [Abstract] [Full Text]  
• Geens, T., Desplanques, A., Van Loock, M., Bonner, B. M., Kaleta, E. F., Magnino, S., Andersen, A. A., Everett, K. D. E., Vanrompay, D. (2005). Sequencing of the Chlamydophila psittaci ompA Gene Reveals a New Genotype, E/B, and the Need for a Rapid Discriminatory Genotyping Method. J. Clin. Microbiol. 43: 2456-2461 [Abstract] [Full Text]  
• Van Loock, M., Vanrompay, D., Herrmann, B., Vander Stappen, J., Volckaert, G., Goddeeris, B. M., Everett, K. D. E. (2003). Missing links in the divergence of Chlamydophila abortus from Chlamydophila psittaci. Int. J. Syst. Evol. Microbiol. 53: 761-770 [Abstract] [Full Text]  
• Rodriguez-Maranon, M. J., Bush, R. M., Peterson, E. M., Schirmer, T., de la Maza, L. M. (2002). Prediction of the membrane-spanning {beta}-strands of the major outer membrane protein of Chlamydia. Protein Sci. 11: 1854-1861 [Abstract] [Full Text]