This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ott, M Articles by Hacker, J Search for Related Content PubMed PubMed Citation Articles by Ott, M Articles by Hacker, J

Pulsed field electrophoresis of genomic restriction fragments for the detection of nosocomial Legionella pneumophila in hospital water supplies.

Institut für Genetik und Mikrobiologie, University of Wurzburg, Germany.

ABSTRACT

Ten Legionella pneumophila strains isolated from different sources were analyzed according to their restriction fragment patterns obtained by cleavage of genomic DNA with NotI and SfiI and separation by pulsed field electrophoresis. Three L. pneumophila isolates from a nosocomial outbreak in Lübeck (Germany) and three other L. pneumophila strains independently isolated from a water tap located in the care unit where the patients were hospitalized exhibited identical restriction fragment profiles. Therefore, we concluded that these environmental specimens were the source of the Legionnaires disease. Another two isolates from patients and two strains from the environment, all unrelated to the outbreak described, showed different cleavage patterns.

This article has been cited by other articles:

• Cordevant, C., Tang, J. S., Cleland, D., Lange, M. (2003). Characterization of Members of the Legionellaceae Family by Automated Ribotyping. J. Clin. Microbiol. 41: 34-43 [Abstract] [Full Text]  
• Jonas, D., Meyer, H.-G. W., Matthes, P., Hartung, D., Jahn, B., Daschner, F. D., Jansen, B. (2000). Comparative Evaluation of Three Different Genotyping Methods for Investigation of Nosocomial Outbreaks of Legionnaires' Disease in Hospitals. J. Clin. Microbiol. 38: 2284-2291 [Abstract] [Full Text]  
• Riffard, S, Lo Presti, F, Vandenesch, F, Forey, F, Reyrolle, M, Etienne, J (1998). Comparative analysis of infrequent-restriction-site PCR and pulsed- field gel electrophoresis for epidemiological typing of Legionella pneumophila serogroup 1 strains [In Process Citation]. J. Clin. Microbiol. 36: 161-167 [Abstract] [Full Text]