This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Endtz, H P Articles by Mouton, R P Search for Related Content PubMed PubMed Citation Articles by Endtz, H P Articles by Mouton, R P

 Previous Article  |  Next Article 

J Clin Microbiol. 1991 May; 29(5): 1007-1010

Comparison of six media, including a semisolid agar, for the isolation of various Campylobacter species from stool specimens.

Department of Medical Microbiology, Leiden University Hospital, The Netherlands.

ABSTRACT

A recently described semisolid blood-free selective motility medium (SSM) (J. Goossens, L. Vlaes, I. Galand, C. Van den Borre, and J. P. Butzler, J. Clin. Microbiol. 27:1077-1080, 1989) was compared with two charcoal-based selective media (charcoal-based selective medium [CSM] and modified charcoal cefoperazone deoxycholate agar [CCDA]), two blood-based media (Skirrow medium [SKM] and CampyBAP), and a passive, 0.65-microns-pore-size cellulose acetate membrane filter technique for the recovery of campylobacters from stools of patients with diarrhea. A total of 1,980 specimens were tested, 161 of which were found to be positive for campylobacters. Campylobacter jejuni was isolated in 148 specimens (91.9%), C. coli was isolated in 27 (7.5%), and "C. upsaliensis" was isolated in 1 (0.6%). After 72 h of incubation with a single medium, the cumulative percentages of Campylobacter-positive specimens isolated on CSM, CCDA, SKM, and SSM were 87, 83, 80, and 72%, respectively. The filter method alone enabled us to recover 61% of all campylobacters. The "C. upsaliensis" strain was isolated by this method only. The highest isolation rates were observed when two media, including CSM, were combined. The combination of CSM and SSM yielded the highest rates (96%), but these were not statistically different from the rates observed with combinations of CSM and SKM (94%) or of CSM and the filter method (91%). Extending the incubation time from 48 to 72 h led to an increase in the isolation rate regardless of the medium used (P less than 0.001). CSM and CCDA were the most selective media. SKM and CampyBAP appeared to be the most inhibitory media for the isolation of C. coli.

This article has been cited by other articles:

• Persson, S., Olsen, K. E. (2005). Multiplex PCR for identification of Campylobacter coli and Campylobacter jejuni from pure cultures and directly on stool samples. J Med Microbiol 54: 1043-1047 [Abstract] [Full Text]  
• Lau, S. K. P., Woo, P. C. Y., Hui, W.-t., Li, M. W. S., Teng, J. L. L., Que, T.-L., Luk, W.-K., Lai, R. W. M., Yung, R. W. H., Yuen, K.-y. (2003). Use of Cefoperazone MacConkey Agar for Selective Isolation of Laribacter hongkongensis. J. Clin. Microbiol. 41: 4839-4841 [Abstract] [Full Text]  
• Hong, Y., Berrang, M. E., Liu, T., Hofacre, C. L., Sanchez, S., Wang, L., Maurer, J. J. (2003). Rapid Detection of Campylobacter coli, C. jejuni, and Salmonella enterica on Poultry Carcasses by Using PCR-Enzyme-Linked Immunosorbent Assay. Appl. Environ. Microbiol. 69: 3492-3499 [Abstract] [Full Text]