This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hamilton, A J Articles by Hay, R J Search for Related Content PubMed PubMed Citation Articles by Hamilton, A J Articles by Hay, R J

Production of species-specific murine monoclonal antibodies against Cryptococcus neoformans which recognize a noncapsular exoantigen.

Department of Clinical Sciences, Guy's Hospital, London, United Kingdom.

ABSTRACT

Three monoclonal antibodies (MAbs), designated 7C5, 7C9, and 5G8, against a cytoplasmic antigen of Cryptococcus neoformans were produced. MAbs 7C5 and 7C9 recognize culture filtrate antigen (exoantigen) of both encapsulated and nonencapsulated isolates of this pathogen, which suggests that they do not recognize capsular polysaccharide material. This is supported by immunofluorescence data which show reactivity of all 3 MAbs to cytoplasm and cell membranes only. MAb 7C9 also recognized C. neoformans var. gattii antigens but no other fungal pathogens tested in an enzyme-linked immunosorbent assay, while 7C5 and 5G8 recognized antigens of the cross-reactive pathogen Trichosporon beigelii but did not recognize either C. neoformans var. gattii isolates or any other fungal antigens. By Western blot (immunoblot), 7C9 detected antigen at 110 to 120, 65 to 70, 45 to 50, and 36 to 38 kDa; in addition to the latter band, the other two MAbs recognized a band at approximately 30 kDa. All three MAbs were of the immunoglobulin G1 subclass. The two MAbs which are capable of reacting with noncapsular culture supernatant antigen have possible uses in serodiagnosis, particularly in AIDS patients infected with C. neoformans, since in this group the present latex agglutination test has some limitations.

This article has been cited by other articles:

• Merkel, G. J., Scofield, B. A. (1999). An Opsonizing Monoclonal Antibody That Recognizes a Noncapsular Epitope Expressed on Cryptococcus neoformans. Infect. Immun. 67: 4994-5000 [Abstract] [Full Text]  
• Hamilton, A. J., Jeavons, L., Youngchim, S., Vanittanakom, N. (1999). Recognition of Fibronectin by Penicillium marneffei Conidia via a Sialic Acid-Dependent Process and Its Relationship to the Interaction Between Conidia and Laminin. Infect. Immun. 67: 5200-5205 [Abstract] [Full Text]  
• Chen, L.-C., Goldman, D. L., Doering, T. L., Pirofski, L.-a., Casadevall, A. (1999). Antibody Response to Cryptococcus neoformans Proteins in Rodents and Humans. Infect. Immun. 67: 2218-2224 [Abstract] [Full Text]  
• Fenelon, L. E., Hamilton, A. J., Figueroa, J. I., Bartholomew, M. A., Allen, M. H., McCarthy, P., Hay, R. J. (1999). Production of Specific Monoclonal Antibodies to Aspergillus Species and Their Use in Immunohistochemical Identification of Aspergillosis. J. Clin. Microbiol. 37: 1221-1223 [Abstract] [Full Text]