This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kain, K C Articles by Lanar, D E Search for Related Content PubMed PubMed Citation Articles by Kain, K C Articles by Lanar, D E

Determination of genetic variation within Plasmodium falciparum by using enzymatically amplified DNA from filter paper disks impregnated with whole blood.

Department of Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.

ABSTRACT

A new method which allows the enzymatic amplification of DNA extracted from whole blood dried on filter paper disks is presented. The method was used to study heterogeneity within an erythrocyte-binding antigen (EBA-175) of Plasmodium falciparum. Blood specimens from malaria-infected patients in Southeast Asia and Africa were spotted onto filter paper, dried, and transported for processing. P. falciparum DNA was extracted by boiling the filter paper disks in the presence of Chelex-100 ion-chelating resin, amplified by the polymerase chain reaction, and analyzed for the presence of genetic variation. In all cases examined, plasmodial DNA was successfully amplified and characterized from filter paper disks. Hybridization of the polymerase chain reaction products with internal probes demonstrated simultaneous infection with two strains of P. falciparum in two patients. This technique represents a sensitive and practical field method for the determination of genetic variation within P. falciparum and the study of molecular epidemiology.

This article has been cited by other articles:

• Osafo-Addo, A. D., Koram, K. A., Oduro, A. R., Wilson, M., Hodgson, A., Rogers, W. O. (2008). HLA-DRB1*04 Allele Is Associated with Severe Malaria in Northern Ghana. Am J Trop Med Hyg 78: 251-255 [Abstract] [Full Text]  
• Banura, C., Franceschi, S., van Doorn, L.-J., Wabwire-Mangen, F., Mbidde, E. K., Weiderpass, E. (2008). Detection of cervical human papillomavirus infection in filter paper samples: a comparative study. J Med Microbiol 57: 253-255 [Full Text]  
• GEBRU-WOLDEAREGAI, T., HAILU, A., GROBUSCH, M. P., KUN, J. F. J. (2005). MOLECULAR SURVEILLANCE OF MUTATIONS IN DIHYDROFOLATE REDUCTASE AND DIHYROPTEROATE SYNTHASE GENES OF PLASMODIUM FALCIPARUM IN ETHIOPIA. Am J Trop Med Hyg 73: 1131-1134 [Abstract] [Full Text]  
• BERECZKY, S., MARTENSSON, A., GIL, J. P., FARNERT, A. (2005). SHORT REPORT: RAPID DNA EXTRACTION FROM ARCHIVE BLOOD SPOTS ON FILTER PAPER FOR GENOTYPING OF PLASMODIUM FALCIPARUM. Am J Trop Med Hyg 72: 249-251 [Abstract] [Full Text]  
• SHIGIDI, M. M. T., HASHIM, R. A., IDRIS, M. N. A., MUKHTAR, M. M., SOKRAB, T.-E. O. (2004). PARASITE DIVERSITY IN ADULT PATIENTS WITH CEREBRAL MALARIA: A HOSPITAL-BASED, CASE-CONTROL STUDY. Am J Trop Med Hyg 71: 754-757 [Abstract] [Full Text]  
• KAILASH, U., HEDAU, S., GOPALKRISHNA, V., KATIYAR, S., DAS, B.C. (2002). A simple 'paper smear' method for dry collection, transport and storage of cervical cytological specimens for rapid screening of HPV infection by PCR. J Med Microbiol 51: 606-683 [Abstract] [Full Text]  
• Zhong, K. J. Y., Salas, C. J., Shafer, R., Gubanov, A., Gasser, R. A. Jr., Magill, A. J., Forney, J. R., Kain, K. C. (2001). Comparison of IsoCode STIX and FTA Gene Guard Collection Matrices as Whole-Blood Storage and Processing Devices for Diagnosis of Malaria by PCR. J. Clin. Microbiol. 39: 1195-1196 [Abstract] [Full Text]  
• Rubio, J. M., Benito, A., Berzosa, P. J., Roche, J., Puente, S., Subirats, M., López-Vélez, R., García, L., Alvar, J. (1999). Usefulness of Seminested Multiplex PCR in Surveillance of Imported Malaria in Spain. J. Clin. Microbiol. 37: 3260-3264 [Abstract] [Full Text]  
• Jarra, W., Snounou, G. (1998). Only Viable Parasites Are Detected by PCR following Clearance of Rodent Malarial Infections by Drug Treatment or Immune Responses. Infect. Immun. 66: 3783-3787 [Abstract] [Full Text]