This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Niki, Y Articles by Armstrong, D Search for Related Content PubMed PubMed Citation Articles by Niki, Y Articles by Armstrong, D

 Previous Article  |  Next Article 

J Clin Microbiol. 1991 July; 29(7): 1317-1322

Model of recurrent pulmonary aspergillosis in rats.

Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

ABSTRACT

Male Sprague-Dawley rats were treated with cortisone acetate and fed a low-protein diet for 3 weeks. At the end of week 2, animals were infected intratracheally with 10(5) conidia of Aspergillus fumigatus H11-20. Despite discontinuation of steroids and the low-protein diet 1 week after the infection, 94% of controls died of invasive pulmonary aspergillosis within 3 weeks postinfection. When rats were treated with a single dose of 1.6 mg of aerosolized amphotericin B per kg of body weight 48 h prior to the infection, mortality was reduced to 11% within 3 weeks postinfection. Despite apparent good health and rapid weight gain, all survivors showed multiple lesions in histopathological sections of the lungs, and 10(3) to 10(4) CFU of aspergilli was recovered from cultures of their lungs. With discontinuation of immunosuppression, the infection was slowly cleared; however, when cortisone acetate was restarted during week 5, reactivation of progressive invasive pulmonary aspergillosis was observed. On the basis of these results, we conclude that a single low dose of aerosolized amphotericin B prophylaxis is effective in preventing an exogenous aspergillus infection of the lung. Additional therapy is needed to prevent recurrent infection caused by endogenous aspergilli when immunosuppression is resumed.

This article has been cited by other articles:

• Ullmann, A. J., Krammes, E., Sommer, S., Buschmann, I., Jahn-Muehl, B., Cacciapuoti, A., Schmitt, H.-J. (2007). Efficacy of posaconazole and amphotericin B in experimental invasive pulmonary aspergillosis in dexamethasone immunosuppressed rats. J Antimicrob Chemother 60: 1080-1084 [Abstract] [Full Text]  
• Takemoto, K., Yamamoto, Y., Ueda, Y., Sumita, Y., Yoshida, K., Niki, Y. (2006). Comparative study on the efficacy of AmBisome and Fungizone in a mouse model of pulmonary aspergillosis. J Antimicrob Chemother 57: 724-731 [Abstract] [Full Text]  
• Takemoto, K., Yamamoto, Y., Ueda, Y., Sumita, Y., Yoshida, K., Niki, Y. (2004). Comparative studies on the efficacy of AmBisome and Fungizone in a mouse model of disseminated aspergillosis. J Antimicrob Chemother 53: 311-317 [Abstract] [Full Text]  
• Martin, M. T., Gavalda, J., Lopez, P., Gomis, X., Ramirez, J. L., Rodriguez, D., Len, O., Jordano, Q., Ruiz, I., Rosal, M., Almirante, B., Pahissa, A. (2003). Efficacy of high doses of liposomal amphotericin B in the treatment of experimental aspergillosis. J Antimicrob Chemother 52: 1032-1034 [Abstract] [Full Text]  
• Latge, J.-P. (1999). Aspergillus fumigatus and Aspergillosis. Clin. Microbiol. Rev. 12: 310-350 [Abstract] [Full Text]