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J Clin Microbiol. 1991 July; 29(7): 1328-1332

Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay.

Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary.

ABSTRACT

Colonies of toxin-producing Pasteurella multocida were detected with peroxidase-labeled monoclonal antibodies by a membrane assay. Examination of the specificity of the assay with 29 P. multocida cultures representing various geographic origins, hosts, and serotypes indicated that the test was specific for toxin-producing strains. No cross-reactions were observed with Bordetella species that can be associated with P. multocida in producing diseases in animals. A single membrane could be used to assay several isolated strains for toxin production or to enumerate toxin-producing colonies in mixed cultures. Toxin-producing P. multocida colonies were detected in primary cultures; hence, the assay appears to have good potential for widespread application with clinical samples.

This article has been cited by other articles:

• Register, K. B., Lee, R. M., Thomson, C. (1998). Two-Color Hybridization Assay for Simultaneous Detection of Bordetella bronchiseptica and Toxigenic Pasteurella multocida from Swine. J. Clin. Microbiol. 36: 3342-3346 [Abstract] [Full Text]