This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Verstijnen, C P Articles by Koanjanart, S Search for Related Content PubMed PubMed Citation Articles by Verstijnen, C P Articles by Koanjanart, S

Enzyme-linked immunosorbent assay using monoclonal antibodies for identification of mycobacteria from early cultures.

Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.

ABSTRACT

A simple enzyme-linked immunosorbent assay (ELISA) for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii has been developed (R. Schöningh, C. P. H. J. Verstijnen, S. Kuijper, and A. H. J. Kolk. J. Clin. Microbiol. 28:708-713, 1990). The test for the routine identification of cultured mycobacteria was introduced in five clinical laboratories located in Tanzania, Thailand, Vietnam, and The Netherlands. The ELISA can be conducted without an ELISA reader since the test can be read visually. The results of identification of 255 strains of the M. tuberculosis complex by microbiological means and by ELISA were compared; the specificity and the sensitivity were 100%. For the M. avium complex, the specificity was 100% and the sensitivity was 64%. All 26 M. kansasii strains tested could be identified as M. kansasii. The ELISA described here proved to be useful in both well- and modestly equipped laboratories and may replace the microbiological method of identification of M. tuberculosis and M. kansasii.

This article has been cited by other articles:

• Pereira Arias-Bouda, L. M., Nguyen, L. N., Ho, L. M., Kuijper, S., Jansen, H. M., Kolk, A. H. J. (2000). Development of Antigen Detection Assay for Diagnosis of Tuberculosis Using Sputum Samples. J. Clin. Microbiol. 38: 2278-2283 [Abstract] [Full Text]