This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fridell, E Articles by Wahren, B Search for Related Content PubMed PubMed Citation Articles by Fridell, E Articles by Wahren, B

 Previous Article  |  Next Article 

J Clin Microbiol. 1991 July; 29(7): 1376-1381

Evaluation of a synthetic-peptide enzyme-linked immunosorbent assay for immunoglobulin M to human parvovirus B19.

Department of Virology, Karolinska Institute, Stockholm, Sweden.

ABSTRACT

A synthetic peptide corresponding to a part of the virus protein 1-virus protein 2 overlapping region of human parvovirus B19 was used in an indirect enzyme-linked immunosorbent assay. Antibodies of the immunoglobulin (Ig) M class were measured in serum samples from patients with erythema infectiosum and controls. In comparison with an IgM assay using native B19 viral antigen, the peptide antigen assay was 92% sensitive and 87% specific. B19 IgM reactivities were seen in a limited number of children with other viral diseases. Specific IgM reactivities to short synthetic viral peptides have previously been reported only with Epstein-Barr virus. Since other sources of viral antigen are limited, the peptide antigen assay may be a useful alternative for the diagnosis of B19-associated disease in human beings.

This article has been cited by other articles:

• Cordoba, P., Lanoel, A., Grutadauria, S., Zapata, M. (2000). Evaluation of Antibodies against a Rubella Virus Neutralizing Domain for Determination of Immune Status. CVI 7: 964-966 [Abstract] [Full Text]  
• Mathiesen, M. J., Christiansen, M., Hansen, K., Holm, A., Åsbrink, E., Theisen, M. (1998). Peptide-Based OspC Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Lyme Borreliosis. J. Clin. Microbiol. 36: 3474-3479 [Abstract] [Full Text]