This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wedege, E Articles by Zollinger, W D Search for Related Content PubMed PubMed Citation Articles by Wedege, E Articles by Zollinger, W D

 Previous Article  |  Next Article 

J Clin Microbiol. 1991 July; 29(7): 1486-1492

Characterization of serogroup A and B strains of Neisseria meningitidis with serotype 4 and 21 monoclonal antibodies and by multilocus enzyme electrophoresis.

Department of Methodology, National Institute of Public Health, Oslo, Norway.

ABSTRACT

The reactions of serogroup A strains of Neisseria meningitidis with one monoclonal antibody specific for serotype 21 and three different monoclonal antibodies specific for serotype 4 were compared with those of serogroup B strains previously assigned to serotype 4. Antibody binding was studied by enzyme-linked immunosorbent assay (ELISA), dot blotting, and immunoblotting. Characterization of the isolates by the electrophoretic mobilities of 14 metabolic enzymes showed 50 multilocus enzyme genotypes. All except two genotypes fell into three distinct clusters: I, IIa and IIb. The enzyme genotypes of serogroup B strains were mainly in cluster I, and 88% of the serogroup A strains had genotypes in clusters IIa and IIb. Serogroup B strains generally reacted with all three serotype 4 monoclonal antibodies in ELISA and dot blotting but with only two in immunoblots. Serogroup A strains showed two different reactions in the blotting methods: either binding of the serotype 21 antibody only or binding of this and two of the three serotype 4 monoclonal antibodies. Strains of the first pattern were in clusters I and IIa, whereas all but two strains in cluster IIb were of the second pattern. In ELISA, an additional reaction of two of the serotype 4 monoclonal antibodies with serogroup A isolates was observed. The different binding of these two monoclonal antibodies in ELISA and the blotting methods appeared to result from heat inactivation of the meningococcal cells and use of detergent-containing reagents in ELISA. The results show that the serotype of serogroup A strains is distinct from serotype 4 of serogroup B strains.

This article has been cited by other articles:

• Norheim, G., Aseffa, A., Yassin, M. A., Mengistu, G., Kassu, A., Fikremariam, D., Tamire, W., Merid, Y., Hoiby, E. A., Caugant, D. A., Fritzsonn, E., Tangen, T., Melak, B., Berhanu, D., Harboe, M., Kolberg, J., Rosenqvist, E. (2008). Specificity of Subcapsular Antibody Responses in Ethiopian Patients following Disease Caused by Serogroup A Meningococci. CVI 15: 863-871 [Abstract] [Full Text]  
• Norheim, G., Aseffa, A., Yassin, M. A., Mengistu, G., Kassu, A., Fikremariam, D., Tamire, W., Merid, Y., Hoiby, E. A., Caugant, D. A., Fritzsonn, E., Tangen, T., Alebel, T., Berhanu, D., Harboe, M., Rosenqvist, E. (2007). Serum Antibody Responses in Ethiopian Meningitis Patients Infected with Neisseria meningitidis Serogroup A Sequence Type 7. CVI 14: 451-463 [Abstract] [Full Text]  
• Urwin, R., Fox, A. J., Musilek, M., Kriz, P., Maiden, M. C. J. (1998). Heterogeneity of the PorB Protein in Serotype 22 Neisseria meningitidis. J. Clin. Microbiol. 36: 3680-3682 [Abstract] [Full Text]