This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Puccia, R Articles by Travassos, L R Search for Related Content PubMed PubMed Citation Articles by Puccia, R Articles by Travassos, L R

43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplasmosis, or Jorge Lobo's disease.

Disciplina de Biologia Celular, Escola Paulista de Medicina, São Paulo, Brazil.

ABSTRACT

Sera from patients with paracoccidioidomycosis (PCM), histoplasmosis (HP), or Jorge Lobo's disease (JL) were titrated against purified gp43 from Paracoccidioides brasiliensis by using both enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation (IPP) reactions with 125I-labeled antigens. In IPP, PCM sera and other sera could be distinguished on the basis of serum titers, whereas in ELISA, 53% of the HP sera and 29% of the JL sera reacted similarly to the PCM sera. To investigate the possible role of the carbohydrate epitopes in these reactions, we compared the reactivities of sera from several patients with native and deglycosylated gp43. Competition experiments were carried out with monosaccharides as inhibitors. The results suggest that greater than 85% of the reactions of the PCM sera with gp43 involved peptide epitopes. Cross-reactions with HP and JL sera in ELISA were predominantly attributed to periodate-sensitive carbohydrate epitopes containing galactosyl residues. HP and JL sera which reacted strongly with gp43 in ELISA were only weakly reactive or did not react in IPP with labeled antigens in solution. Moreover, ELISA reactions could be significantly inhibited either by monosaccharides or by periodate treatment. Apparently, carbohydrate epitopes in gp43 are more accessible to the antibodies when the molecule is bound to a plastic substrate than when it is in solution. Structural changes in the gp43 antigen arising by N deglycosylation abolish reactivity with PCM sera and support the existence of conformational peptide epitopes.

This article has been cited by other articles:

• Carvalho, K. C., Vallejo, M. C., Camargo, Z. P., Puccia, R. (2008). Use of Recombinant gp43 Isoforms Expressed in Pichia pastoris for Diagnosis of Paracoccidioidomycosis. CVI 15: 622-629 [Abstract] [Full Text]  
• Mendoza, L., Belone, A. F. F., Vilela, R., Rehtanz, M., Bossart, G. D., Reif, J. S., Fair, P. A., Durden, W. N., St. Leger, J., Travassos, L. R., Rosa, P. S. (2008). Use of Sera from Humans and Dolphins with Lacaziosis and Sera from Experimentally Infected Mice for Western Blot Analyses of Lacazia loboi Antigens. CVI 15: 164-167 [Abstract] [Full Text]  
• Iwai, L. K., Yoshida, M., Sadahiro, A., da Silva, W. R., Marin, M. L., Goldberg, A. C., Juliano, M. A., Juliano, L., Shikanai-Yasuda, M. A., Kalil, J., Cunha-Neto, E., Travassos, L. R. (2007). T-Cell Recognition of Paracoccidioides brasiliensis gp43-Derived Peptides in Patients with Paracoccidioidomycosis and Healthy Individuals. CVI 14: 474-476 [Abstract] [Full Text]  
• Bertini, S., Colombo, A. L., Takahashi, H. K., Straus, A. H. (2007). Expression of Antibodies Directed to Paracoccidioides brasiliensis Glycosphingolipids during the Course of Paracoccidioidomycosis Treatment. CVI 14: 150-156 [Abstract] [Full Text]  
• Pitarch, A., Jimenez, A., Nombela, C., Gil, C. (2006). Decoding Serological Response to Candida Cell Wall Immunome into Novel Diagnostic, Prognostic, and Therapeutic Candidates for Systemic Candidiasis by Proteomic and Bioinformatic Analyses. Mol. Cell. Proteomics 5: 79-96 [Abstract] [Full Text]  
• Vilela, R., Mendoza, L., Rosa, P. S., Belone, A. F. F., Madeira, S., Opromolla, D. V. A., de Resende, M. A. (2005). Molecular Model for Studying the Uncultivated Fungal Pathogen Lacazia loboi. J. Clin. Microbiol. 43: 3657-3661 [Abstract] [Full Text]  
• Vidal, M. S. M., Benard, G., de Brito, T., Dantas, K. C., Pereira, C. N., Franca, F. O. S., da Silva, A. M. G., Martins, J. E. C. (2005). Atypical Serological Response Marked by a Lack of Detectable Anti-gp43 Antibodies in a Patient with Disseminated Paracoccidioidomycosis. J. Clin. Microbiol. 43: 3014-3016 [Abstract] [Full Text]  
• Albuquerque, C. F., Silva, S. H. M. d., Camargo, Z. P. (2005). Improvement of the Specificity of an Enzyme-Linked Immunosorbent Assay for Diagnosis of Paracoccidioidomycosis. J. Clin. Microbiol. 43: 1944-1946 [Abstract] [Full Text]  
• Berzaghi, R., Marques da Silva, S. H., Pires de Camargo, Z. (2005). Variable gp43 Secretion by Paracoccidioides brasiliensis Clones Obtained by Two Different Culture Methods. J. Clin. Microbiol. 43: 491-493 [Abstract] [Full Text]  
• Marques da Silva, S. H., de Mattos Grosso, D., Lopes, J. D., Colombo, A. L., Blotta, M. H. S. L., Queiroz-Telles, F., Pires de Camargo, Z. (2004). Detection of Paracoccidioides brasiliensis gp70 Circulating Antigen and Follow-Up of Patients Undergoing Antimycotic Therapy. J. Clin. Microbiol. 42: 4480-4486 [Abstract] [Full Text]  
• Marques da Silva, S. H., Queiroz-Telles, F., Colombo, A. L., Blotta, M. H. S. L., Lopes, J. D., Pires de Camargo, Z. (2004). Monitoring gp43 Antigenemia in Paracoccidioidomycosis Patients during Therapy. J. Clin. Microbiol. 42: 2419-2424 [Abstract] [Full Text]  
• Hebeler-Barbosa, F., Morais, F. V., Montenegro, M. R., Kuramae, E. E., Montes, B., McEwen, J. G., Bagagli, E., Puccia, R. (2003). Comparison of the Sequences of the Internal Transcribed Spacer Regions and PbGP43 Genes of Paracoccidioides brasiliensis from Patients and Armadillos (Dasypus novemcinctus). J. Clin. Microbiol. 41: 5735-5737 [Abstract] [Full Text]  
• de Mattos Grosso, D., de Almeida, S. R., Mariano, M., Lopes, J. D. (2003). Characterization of gp70 and Anti-gp70 Monoclonal Antibodies in Paracoccidioides brasiliensis Pathogenesis. Infect. Immun. 71: 6534-6542 [Abstract] [Full Text]  
• Neves, A. R., Mamoni, R. L., Rossi, C. L., de Camargo, Z. P., Blotta, M. H. S. L. (2003). Negative Immunodiffusion Test Results Obtained with Sera of Paracoccidioidomycosis Patients May Be Related to Low-Avidity Immunoglobulin G2 Antibodies Directed against Carbohydrate Epitopes. CVI 10: 802-807 [Abstract] [Full Text]  
• Marques da Silva, S. H., Colombo, A. L., Blotta, M. H. S. L., Lopes, J. D., Queiroz-Telles, F., Pires de Camargo, Z. (2003). Detection of Circulating gp43 Antigen in Serum, Cerebrospinal Fluid, and Bronchoalveolar Lavage Fluid of Patients with Paracoccidioidomycosis. J. Clin. Microbiol. 41: 3675-3680 [Abstract] [Full Text]  
• Diniz, S. N., Carvalho, K. C., Cisalpino, P. S., Silveira, J. F., Travassos, L. R., Puccia, R. (2002). Expression in Bacteria of the Gene Encoding the gp43 Antigen of Paracoccidioides brasiliensis: Immunological Reactivity of the Recombinant Fusion Proteins. CVI 9: 1200-1204 [Abstract] [Full Text]  
• Yeo, S. F., Wong, B. (2002). Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections. Clin. Microbiol. Rev. 15: 465-484 [Abstract] [Full Text]  
• Morais, F. V., Barros, T. F., Fukada, M. K., Cisalpino, P. S., Puccia, R. (2000). Polymorphism in the Gene Coding for the Immunodominant Antigen gp43 from the Pathogenic Fungus Paracoccidioides brasiliensis. J. Clin. Microbiol. 38: 3960-3966 [Abstract] [Full Text]  
• Bialek, R., Ibricevic, A., Aepinus, C., Najvar, L. K., Fothergill, A. W., Knobloch, J., Graybill, J. R. (2000). Detection of Paracoccidioides brasiliensis in Tissue Samples by a Nested PCR Assay. J. Clin. Microbiol. 38: 2940-2942 [Abstract] [Full Text]  
• Souza, A. R., Gesztesi, J.-L., del Negro, G. M. B., Benard, G., Sato, J., Santos, M. V. B., Abrahao, T. B., Lopes, J. D. (2000). Anti-Idiotypic Antibodies in Patients with Different Clinical Forms of Paracoccidioidomycosis. CVI 7: 175-181 [Abstract] [Full Text]  
• Taborda, C. P., Juliano, M. A., Puccia, R., Franco, M., Travassos, L. R. (1998). Mapping of the T-Cell Epitope in the Major 43-Kilodalton Glycoprotein of Paracoccidioides brasiliensis Which Induces a Th-1 Response Protective against Fungal Infection in BALB/c Mice. Infect. Immun. 66: 786-793 [Abstract] [Full Text]  
• Cisalpino, P.íc. S., Puccia, R., Yamauchi, L. M., Cano, M. I. N., Silveira, J. F. d., Travassos, L. R. (1996). Cloning, Characterization, and Epitope Expression of the Major Diagnostic Antigen of Paracoccidioides brasiliensis. J. Biol. Chem. 271: 4553-4560 [Abstract] [Full Text]