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J Clin Microbiol. 1991 August; 29(8): 1652-1658

Mycobacterium paratuberculosis antigen D: characterization and evidence that it is a bacterioferritin.

Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario, Canada.

ABSTRACT

By using a combination of agarose and polyacrylamide gel electrophoresis, Mycobacterium paratuberculosis antigen D was resolved from a crude sonicated preparation of the organism and characterized as a component with a molecular mass of approximately 400,000 Da. While this component was composed mainly of protein, with unusually high proportions of glutamic acid and leucine, it was resistant to digestion with a number of proteolytic enzymes. Structural detail revealed by electron microscopy, amino acid sequence data, and the demonstration of a Soret band in its absorption spectrum indicated that antigen D was similar to an Escherichia coli bacterioferritin.

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