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J Clin Microbiol. 1976 June; 3(6): 604-608

Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test.

G C Saunders and E H Clinard

ABSTRACT

We have developed a rapid and simple indirect enzyme-labeled antibody procedure suitable for screening for antibodies to viral, bacterial, and parasitic antigens. Disposable microplates were utilized as antigen carriers and test vehicles. The test worked equally well with serum or whole unclotted blood. Using an automatic pipettor, 96 tests could be performed in 30 to 60 min. We report on the development of enzyme-labeled antibody procedures for the detection and surveillance of animal diseases.

J Clin Microbiol. 1976 June; 3(6): 604-608






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1976 by the American Society for Microbiology. All rights reserved.