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J Clin Microbiol. 1976 June; 3(6): 626-631

Large-scale purification of hepatitis B surface antigen.

J Vnek and A M Prince

ABSTRACT

Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02 M sodium phosphate buffer.

J Clin Microbiol. 1976 June; 3(6): 626-631






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1976 by the American Society for Microbiology. All rights reserved.