This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zwaagstra, K A Articles by Kusters, J G Search for Related Content PubMed PubMed Citation Articles by Zwaagstra, K A Articles by Kusters, J G

Rapid detection and identification of avian infectious bronchitis virus.

Department of Bacteriology, School of Veterinary Medicine, University of Utrecht, The Netherlands.

ABSTRACT

A rapid and sensitive method for the detection and unambiguous typing of infectious bronchitis virus (IBV) is described. RNA was isolated from IBV-infected allantoic fluid and was transcribed into cDNA. This cDNA was amplified by the polymerase chain reaction. The polymerase chain reaction products were subsequently analyzed on an agarose gel. The presence of IBV-specific RNA in the allantoic fluid then allowed the amplification of a 438-bp DNA fragment from the nucleocapsid (N) gene. For the typing of IBV isolates, we used amplified double-stranded DNA as a template in a sequencing reaction. We report 360 bases of the N gene of 18 IBV isolates. The sequence of the N gene was different between serologically indistinguishable IBV strains and may be a valuable tool in epidemiologic studies. A phylogenetic tree that was based on the sequences obtained did not agree with trees that were based on other parts of the sequence, illustrating the high frequency of recombination between IBV strains.

This article has been cited by other articles:

• Mardani, K., Noormohammadi, A. H., Hooper, P., Ignjatovic, J., Browning, G. F. (2008). Infectious Bronchitis Viruses with a Novel Genomic Organization. J. Virol. 82: 2013-2024 [Abstract] [Full Text]  
• Bayry, J., Goudar, M. S., Nighot, P. K., Kshirsagar, S. G., Ladman, B. S., Gelb, J. Jr., Ghalsasi, G. R., Kolte, G. N. (2005). Emergence of a Nephropathogenic Avian Infectious Bronchitis Virus with a Novel Genotype in India. J. Clin. Microbiol. 43: 916-918 [Abstract] [Full Text]  
• He, Q., Chong, K. H., Chng, H. H., Leung, B., Ling, A. E., Wei, T., Chan, S.-W., Ooi, E. E., Kwang, J. (2004). Development of a Western Blot Assay for Detection of Antibodies against Coronavirus Causing Severe Acute Respiratory Syndrome. CVI 11: 417-422 [Abstract] [Full Text]