This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gerna, G Articles by Flores, J Search for Related Content PubMed PubMed Citation Articles by Gerna, G Articles by Flores, J

 Previous Article  |  Next Article 

J Clin Microbiol. 1992 January; 30(1): 9-16

Isolation and characterization of two distinct human rotavirus strains with G6 specificity.

Institute of Infectious Diseases, University of Pavia, Italy.

ABSTRACT

Two new human rotavirus (HRV) strains, PA151 and PA169, with subgroup I specificity and a long RNA pattern, yet with a serotype G (VP7) specificity different from those of any of the six well-established HRV serotypes (G1 to G4, G8, and G9), were isolated 3 months apart from two children with acute gastroenteritis in Sicily, southern Italy, in the winter season of 1987 and 1988. The HRV isolates were adapted to growth in cell cultures and were then characterized by neutralization and RNA-RNA (Northern blot) hybridization. Cross-neutralization studies with type-specific immune sera to RV serotypes 1 to 10 showed the antigenic relatedness of the two strains with serotype 6 bovine strains UK and NCDV. Monoclonal antibodies to VP7 of UK were able to recognize UK and NCDV strains as well as both HRV isolates. Cross-hybridization studies showed a genetic relatedness of PA151 and PA169 to bovine strains for all genes except gene 4. Gene 4 of PA151 appeared to be genetically related to that of AU228 (a human strain of subgroup I and with serotype G3 specificity that belongs to a feline genogroup), whereas gene 4 of PA169 appeared to be unique, yet it was related to gene 4 of two recently reported subgroup I HRV strains, one (PA710) with serotype G3 specificity and the other (HAL1271) with serotype G8 specificity. The new HRV strains must be taken into consideration when deciding strategies for the development of an effective RV vaccine.

This article has been cited by other articles:

• Gulati, B. R., Deepa, R., Singh, B. K., Rao, C. D. (2007). Diversity in Indian Equine Rotaviruses: Identification of Genotype G10,P6[1] and G1 Strains and a New VP7 Genotype (G16) Strain in Specimens from Diarrheic Foals in India. J. Clin. Microbiol. 45: 972-978 [Abstract] [Full Text]  
• Park, S.-H., Saif, L. J., Jeong, C., Lim, G.-K., Park, S.-I., Kim, H.-H., Park, S.-J., Kim, Y.-J., Jeong, J.-H., Kang, M.-I., Cho, K.-O. (2006). Molecular Characterization of Novel G5 Bovine Rotavirus Strains. J. Clin. Microbiol. 44: 4101-4112 [Abstract] [Full Text]  
• Matthijnssens, J., Rahman, M., Martella, V., Xuelei, Y., De Vos, S., De Leener, K., Ciarlet, M., Buonavoglia, C., Van Ranst, M. (2006). Full genomic analysis of human rotavirus strain b4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission.. J. Virol. 80: 3801-3810 [Abstract] [Full Text]  
• van der Heide, R., Koopmans, M. P. G., Shekary, N., Houwers, D. J., van Duynhoven, Y. T. H. P., van der Poel, W. H. M. (2005). Molecular Characterizations of Human and Animal Group A Rotaviruses in The Netherlands. J. Clin. Microbiol. 43: 669-675 [Abstract] [Full Text]  
• Banyai, K., Gentsch, J. R., Glass, R. I., Uj, M., Mihaly, I., Szucs, G. (2004). Eight-Year Survey of Human Rotavirus Strains Demonstrates Circulation of Unusual G and P Types in Hungary. J. Clin. Microbiol. 42: 393-397 [Abstract] [Full Text]  
• Martella, V., Ciarlet, M., Pratelli, A., Arista, S., Terio, V., Elia, G., Cavalli, A., Gentile, M., Decaro, N., Greco, G., Cafiero, M. A., Tempesta, M., Buonavoglia, C. (2003). Molecular Analysis of the VP7, VP4, VP6, NSP4, and NSP5/6 Genes of a Buffalo Rotavirus Strain: Identification of the Rare P[3] Rhesus Rotavirus-Like VP4 Gene Allele. J. Clin. Microbiol. 41: 5665-5675 [Abstract] [Full Text]  
• Rahman, M., De Leener, K., Goegebuer, T., Wollants, E., Van der Donck, I., Hoovels, L. V., Van Ranst, M. (2003). Genetic Characterization of a Novel, Naturally Occurring Recombinant Human G6P[6] Rotavirus. J. Clin. Microbiol. 41: 2088-2095 [Abstract] [Full Text]  
• El-Attar, L., Dhaliwal, W., Iturriza-Gomara, M., Bridger, J. C. (2002). Identification and Molecular Characterization of a Bovine G3 Rotavirus Which Causes Age-Independent Diarrhea in Cattle. J. Clin. Microbiol. 40: 937-942 [Abstract] [Full Text]  
• Adah, M. I., Wade, A., Taniguchi, K. (2001). Molecular Epidemiology of Rotaviruses in Nigeria: Detection of Unusual Strains with G2P[6] and G8P[1] Specificities. J. Clin. Microbiol. 39: 3969-3975 [Abstract] [Full Text]  
• COONEY, M.A., GORRELL, R.J., PALOMBO, E.A. (2001). Characterisation and phylogenetic analysis of the VP7 proteins of serotype G6 and G8 human rotaviruses. J Med Microbiol 50: 462-467 [Abstract] [Full Text]  
• Delorme, C., Brüssow, H., Sidoti, J., Roche, N., Karlsson, K.-A., Neeser, J.-R., Teneberg, S. (2001). Glycosphingolipid Binding Specificities of Rotavirus: Identification of a Sialic Acid-Binding Epitope. J. Virol. 75: 2276-2287 [Abstract] [Full Text]  
• Martella, V., Pratelli, A., Greco, G., Tempesta, M., Ferrari, M., Losio, M. N., Buonavoglia, C. (2001). Genomic Characterization of Porcine Rotaviruses in Italy. CVI 8: 129-132 [Abstract] [Full Text]  
• Frühwirth, M., Brösl, S., Ellemunter, H., Moll-Schüler, I., Rohwedder, A., Mutz, I. (2000). Distribution of Rotavirus VP4 Genotypes and VP7 Serotypes among Nonhospitalized and Hospitalized Patients with Gastroenteritis and Patients with Nosocomially Acquired Gastroenteritis in Austria. J. Clin. Microbiol. 38: 1804-1806 [Abstract] [Full Text]  
• Chang, K. O., Nielsen, P. R., Ward, L. A., Saif, L. J. (1999). Dual Infection of Gnotobiotic Calves with Bovine Strains of Group A and Porcine-Like Group C Rotaviruses Influences Pathogenesis of the Group C Rotavirus. J. Virol. 73: 9284-9293 [Abstract] [Full Text]  
• Steele, A. D., Parker, S. P., Peenze, I., Pager, C. T., Taylor, M. B., Cubitt, W. D. (1999). Comparative studies of human rotavirus serotype G8 strains recovered in South Africa and the United Kingdom. J. Gen. Virol. 80: 3029-3034 [Abstract] [Full Text]  
• Arista, S., Vizzi, E., Alaimo, C., Palermo, D., Cascio, A. (1999). Identification of Human Rotavirus Strains with the P[14] Genotype by PCR. J. Clin. Microbiol. 37: 2706-2708 [Abstract] [Full Text]  
• Ramachandran, M., Gentsch, J. R., Parashar, U. D., Jin, S., Woods, P. A., Holmes, J. L., Kirkwood, C. D., Bishop, R. F., Greenberg, H. B., Urasawa, S., Gerna, G., Coulson, B. S., Taniguchi, K., Bresee, J. S., Glass, R. I., the National Rotavirus Strain Surveillance System, (1998). Detection and Characterization of Novel Rotavirus Strains in the United States. J. Clin. Microbiol. 36: 3223-3229 [Abstract] [Full Text]