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J Clin Microbiol. 1992 November; 30(11): 2772-2775

Genetic heterogeneity within Mycobacterium fortuitum complex species: genotypic criteria for identification.

P Kirschner, M Kiekenbeck, D Meissner, J Wolters and E C Böttger

Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.

ABSTRACT

A 1.5-kb segment of the DNA that encodes 16S rRNA was amplified by polymerase chain reaction, and 880 nucleotide positions were determined from each of the described biovariants of Mycobacterium fortuitum. Signature sequences which allow rapid identification of M. fortuitum strains at the biovariant level are described. Our data demonstrate a close phylogenetic relationship between Mycobacterium senegalense and M. fortuitum and indicate that the described biovariants of M. fortuitum represent genetically distinct taxa.


J Clin Microbiol. 1992 November; 30(11): 2772-2775




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