This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Loeffelholz, M J Articles by Dragon, E A Search for Related Content PubMed PubMed Citation Articles by Loeffelholz, M J Articles by Dragon, E A

 Previous Article  |  Next Article 

J Clin Microbiol. 1992 November; 30(11): 2847-2851

Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction.

Roche Molecular Systems, Branchburg, New Jersey 08876-1760.

ABSTRACT

A rapid and sensitive polymerase chain reaction (PCR)-based assay for detection of Chlamydia trachomatis in cervical specimens is described. This assay consists of (i) sample preparation which avoids the use of heat, centrifugation, or organic extractions; (ii) rapid, two-temperature PCR amplification of C. trachomatis cryptic plasmid sequences; and (iii) capture and colorimetric detection of amplified DNA in microwell plates. PCR was compared with culture by using 503 cervical specimens. After resolution of discrepant specimens with a confirmatory PCR assay directed against the chlamydial major outer membrane protein gene, PCR had a sensitivity of 97% and a specificity of 99.7% while culture had a sensitivity of 85.7% and a specificity of 100%. In a separate study, PCR was compared with a direct specimen enzyme immunoassay (Chlamydiazyme; Abbott Diagnostics) by using 375 cervical specimens. After resolution of discrepant specimens, PCR had a sensitivity and specificity of 100%, while the enzyme immunoassay had a sensitivity of 58.8% and a specificity of 100%.

This article has been cited by other articles:

• Yin, Y-P, Peeling, R W, Chen, X-S, Gong, K-L, Zhou, H, Gu, W-M, Zheng, H-P, Wang, Z-S, Yong, G, Cao, W-L, Shi, M-Q, Wei, W-H, Dai, X-Q, Gao, X, Chen, Q, Mabey, D (2006). Clinic-based evaluation of Clearview Chlamydia MF for detection of Chlamydia trachomatis in vaginal and cervical specimens from women at high risk in China. Sex. Transm. Infect. 82: v33-v37 [Abstract] [Full Text]  
• Horner, P., Skidmore, S., Herring, A., Sell, J., Paul, I., Caul, O., Egger, M., McCarthy, A., Sanford, E., Salisbury, C., MacLeod, J., Sterne, J., Low, N., for the Chlamydia Screening Studies (ClaSS) Group, (2005). Enhanced Enzyme Immunoassay with Negative-Gray-Zone Testing Compared to a Single Nucleic Acid Amplification Technique for Community-Based Chlamydial Screening of Men. J. Clin. Microbiol. 43: 2065-2069 [Abstract] [Full Text]  
• WATSON, E. J., TEMPLETON, A., RUSSELL, I., PAAVONEN, J., MARDH, P.-A., STARY, A., PEDERSON, B. S. (2002). The accuracy and efficacy of screening tests for Chlamydia trachomatis: a systematic review. J Med Microbiol 51: 1021-1031 [Abstract] [Full Text]  
• Bianchi, A., Moret, F., Desrues, J.-M., Champenois, T., Dervaux, Y., Desvouas, O., Oursin, A., Quinzat, D., Dachez, R., Bathelier, C., Ronsin, C. (2002). PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a Suitable Medium for Detection of Chlamydia trachomatis by the COBAS Amplicor CT/NG Test: Results of a Preliminary Study and Future Implications. J. Clin. Microbiol. 40: 1749-1754 [Abstract] [Full Text]  
• Aslanzadeh, J., Jones, M. (2002). Comparison of M4 and M4RT Media for Transporting Cervical Swab Samples for PCR Detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Annals of Clinical & Laboratory Science 32: 61-64 [Abstract] [Full Text]  
• Van Dyck, E., Ieven, M., Pattyn, S., Van Damme, L., Laga, M. (2001). Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Enzyme Immunoassay, Culture, and Three Nucleic Acid Amplification Tests. J. Clin. Microbiol. 39: 1751-1756 [Abstract] [Full Text]  
• Lee, S. C., Antony, A., Lee, N., Leibow, J., Yang, J. Q., Soviero, S., Gutekunst, K., Rosenstraus, M. (2000). Improved Version 2.0 Qualitative and Quantitative AMPLICOR Reverse Transcription-PCR Tests for Hepatitis C Virus RNA: Calibration to International Units, Enhanced Genotype Reactivity, and Performance Characteristics. J. Clin. Microbiol. 38: 4171-4179 [Abstract] [Full Text]  
• Winter, A. J., Gilleran, G., Eastick, K., Ross, J. D. C. (2000). Comparison of a Ligase Chain Reaction-Based Assay and Cell Culture for Detection of Pharyngeal Carriage of Chlamydia trachomatis. J. Clin. Microbiol. 38: 3502-3504 [Abstract] [Full Text]  
• Tanaka, M., Nakayama, H., Sagiyama, K., Haraoka, M., Yoshida, H., Hagiwara, T., Akazawa, K., Naito, S. (2000). Evaluation of a new amplified enzyme immunoassay (EIA) for the detection of Chlamydia trachomatis in male urine, female endocervical swab, and patient obtained vaginal swab specimens. J. Clin. Pathol. 53: 350-354 [Abstract] [Full Text]  
• IRWIN, K. L., MOORMAN, A. C., O'SULLIVAN, M. J., SPERLING, R., KOESTLER, M. E., SOTO, I., RICE, R., BRODMAN, M., YASIN, S., DROESE, A., ZHANG, D., SCHWARTZ, D. A., BYERS, R. H. (2000). Influence of Human Immunodeficiency Virus Infection on Pelvic Inflammatory Disease. Obstet Gynecol 95: 525-534 [Abstract] [Full Text]  
• Van Der Pol, B., Quinn, T. C., Gaydos, C. A., Crotchfelt, K., Schachter, J., Moncada, J., Jungkind, D., Martin, D. H., Turner, B., Peyton, C., Jones, R. B. (2000). Multicenter Evaluation of the AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Detection of Chlamydia trachomatis. J. Clin. Microbiol. 38: 1105-1112 [Abstract] [Full Text]  
• Tang, Y.-W., Mitchell, P. S., Espy, M. J., Smith, T. F., Persing, D. H. (1999). Molecular Diagnosis of Herpes Simplex Virus Infections in the Central Nervous System. J. Clin. Microbiol. 37: 2127-2136 [Full Text]  
• Matsumoto, A., Izutsu, H., Miyashita, N., Ohuchi, M. (1998). Plaque Formation by and Plaque Cloning of Chlamydia trachomatis Biovar Trachoma. J. Clin. Microbiol. 36: 3013-3019 [Abstract] [Full Text]  
• Belgrader, P., Benett, W., Hadley, D., Long, G., Mariella, R. Jr., Milanovich, F., Nasarabadi, S., Nelson, W., Richards, J., Stratton, P. (1998). Rapid pathogen detection using a microchip PCR array instrument. Clin. Chem. 44: 2191-2194 [Abstract] [Full Text]  
• Long, C. M., Drew, L., Miner, R., Jekic-McMullen, D., Impraim, C., Kao, S.-Y. (1998). Detection of Cytomegalovirus in Plasma and Cerebrospinal Fluid Specimens from Human Immunodeficiency Virus-Infected Patients by the AMPLICOR CMV Test. J. Clin. Microbiol. 36: 2434-2438 [Abstract] [Full Text]  
• Tang, Y.-W., Rys, P. N., Rutledge, B. J., Mitchell, P. S., Smith, T. F., Persing, D. H. (1998). Comparative Evaluation of Colorimetric Microtiter Plate Systems for Detection of Herpes Simplex Virus in Cerebrospinal Fluid. J. Clin. Microbiol. 36: 2714-2717 [Abstract] [Full Text]  
• Toye, B., Woods, W., Bobrowska, M., Ramotar, K. (1998). Inhibition of PCR in Genital and Urine Specimens Submitted for Chlamydia trachomatis Testing. J. Clin. Microbiol. 36: 2356-2358 [Abstract] [Full Text]  
• Mulcahy, G. M., Albanese, E. A., Bachl, B. L. (1998). Reproducibility of the Roche Amplicor Polymerase Chain Reaction Assay for Detection of Infection by Chlamydia trachomatis in Endocervical Specimens. Clin. Chem. 44: 1575-1578 [Full Text]  
• Stauffer, F., Haber, H., Rieger, A., Mutschlechner, R., Hasenberger, P., Tevere, V. J., Young, K. K. Y. (1998). Genus Level Identification of Mycobacteria from Clinical Specimens by Using an Easy-To-Handle Mycobacterium-Specific PCR Assay. J. Clin. Microbiol. 36: 614-617 [Abstract] [Full Text]  
• Green, T. A., Black, C. M., Johnson, R. E. (1998). Evaluation of Bias in Diagnostic-Test Sensitivity and Specificity Estimates Computed by Discrepant Analysis. J. Clin. Microbiol. 36: 375-381 [Abstract] [Full Text]  
• Dean, D, Ferrero, D, McCarthy, M (1998). Comparison of performance and cost-effectiveness of direct fluorescent- antibody, ligase chain reaction, and PCR assays for verification of chlamydial enzyme immunoassay results for populations with a low to moderate prevalence of Chlamydia trachomatis infection [In Process Citation]. J. Clin. Microbiol. 36: 94-99 [Abstract] [Full Text]  
• Rosenstraus, M, Wang, Z, Chang, S., DeBonville, D, Spadoro, J. (1998). An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance [In Process Citation]. J. Clin. Microbiol. 36: 191-197 [Abstract] [Full Text]  
• Tang, Y.-W., Procop, G. W., Persing, D. H. (1997). Molecular diagnostics of infectious diseases. Clin. Chem. 43: 2021-2038 [Abstract] [Full Text]