This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Talley, N J Articles by Zinsmeister, A R Search for Related Content PubMed PubMed Citation Articles by Talley, N J Articles by Zinsmeister, A R

Comparison of commercial serological tests for detection of Helicobacter pylori antibodies.

Gastroenterology Unit, Mayo Clinic, Rochester, Minnesota 55905.

ABSTRACT

Serological testing for Helicobacter pylori infection is one of the diagnostic methods of choice. Various commercial kits that use different antigens have been developed, but data on their diagnostic accuracy and direct comparisons between the tests are lacking. We aimed to evaluate the sensitivity, specificity, and predictive value of three immunoglobulin G enzyme-linked immunosorbent assay kits: Pylori stat, Helico-G, and Premier H. pylori. Serum samples and gastric biopsy findings from 76 patients were evaluated. We found by using a priori cutoff values that the Pylori stat, Helico-G, and Premier kits had overall sensitivities of 96, 96, and 88%, respectively, and specificities of 94, 86, and 96%, respectively, compared with gastric biopsy findings. For 232 serum samples, the Pylori stat test and a previously validated standard serological assay on which the test was based disagreed in 3% of the cases, while for 76 samples that were tested, Helico-G and a previously validated standard assay on which it was based disagreed in 8% of the cases. The intra- and interassay precision of each of the test kits was high. We conclude that serology based on any of these commercial tests represents a reliable and valid method for the diagnosis of H. pylori whether or not highly purified antigens are used.

This article has been cited by other articles:

• Sheehan, J, Kearney, P M, Sullivan, S O, Mongan, C, Kelly, E, Perry, I J (2005). Acute coronary syndrome and chronic infection in the Cork coronary care case-control study. Heart 91: 19-22 [Abstract] [Full Text]  
• Fendrick, A. M., Chernew, M. E., Hirth, R. A., Bloom, B. S., Bandekar, R. R., Scheiman, J. M. (1999). Clinical and Economic Effects of Population-Based Helicobacter pylori Screening to Prevent Gastric Cancer. Arch Intern Med 159: 142-148 [Abstract] [Full Text]  
• Laheij, R. J. F., Straatman, H., Jansen, J. B. M. J., Verbeek, A. L. M. (1998). Evaluation of Commercially Available Helicobacter pylori Serology Kits: a Review. J. Clin. Microbiol. 36: 2803-2809 [Full Text]  
• Kinoshita, Y, Kawanami, C, Kishi, K, Nakata, H, Seino, Y, Chiba, T (1997). Helicobacter pylori independent chronological change in gastric acid secretion in the Japanese. Gut 41: 452-458 [Abstract] [Full Text]