This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wicher, K Articles by Wicher, V Search for Related Content PubMed PubMed Citation Articles by Wicher, K Articles by Wicher, V

 Previous Article  |  Next Article 

J Clin Microbiol. 1992 February; 30(2): 497-500

Detection of Treponema pallidum in early syphilis by DNA amplification.

Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.

ABSTRACT

By using experimentally infected rabbits as a model for early syphilis, the applicability of in vitro DNA amplification was explored for detection of Treponema pallidum. It was determined that whole blood in heparin or EDTA (but not serum), lesion exudate, and punch biopsy as well as swabs of lesions are useful specimens for examination by the polymerase chain reaction. Swabs do not require special diluents, and the specimens, whether kept at room temperature or frozen, are well suited for use in the polymerase chain reaction.

This article has been cited by other articles:

• Michelow, I. C., Wendel, G. D. Jr., Norgard, M. V., Zeray, F., Leos, N. K., Alsaadi, R., Sanchez, P. J. (2002). Central Nervous System Infection in Congenital Syphilis. NEJM 346: 1792-1798 [Abstract] [Full Text]  
• Liu, H., Rodes, B., Chen, C.-Y., Steiner, B. (2001). New Tests for Syphilis: Rational Design of a PCR Method for Detection of Treponema pallidum in Clinical Specimens Using Unique Regions of the DNA Polymerase I Gene. J. Clin. Microbiol. 39: 1941-1946 [Abstract] [Full Text]  
• Wicher, K., Wicher, V., Abbruscato, F., Baughn, R. E. (2000). Treponema pallidum subsp. pertenue Displays Pathogenic Properties Different from Those of T. pallidum subsp. pallidum. Infect. Immun. 68: 3219-3225 [Abstract] [Full Text]  
• Villanueva, A. V., Podzorski, R. P., Reyes, M. P. (1998). Effects of Various Handling and Storage Conditions on Stability of Treponema pallidum DNA in Cerebrospinal Fluid. J. Clin. Microbiol. 36: 2117-2119 [Abstract] [Full Text]  
• Wicher, K., Abbruscato, F., Wicher, V., Collins, D. N., Auger, I., Horowitz, H. W. (1998). Identification of Persistent Infection in Experimental Syphilis by PCR. Infect. Immun. 66: 2509-2513 [Abstract] [Full Text]  
• Horowitz, H. W., Valsamis, M. P., Wicher, V., Abbruscato, F., Larsen, S. A., Wormser, G. P., Wicher, K. (1994). Cerebral Syphilitic Gumma Confirmed by the Polymerase Chain Reaction in a Man with Human Immunodeficiency Virus Infection. NEJM 331: 1488-1491 [Full Text]