This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hughes, C A Articles by Johnson, R C Search for Related Content PubMed PubMed Citation Articles by Hughes, C A Articles by Johnson, R C

DNA analysis of Borrelia burgdorferi NCH-1, the first northcentral U.S. human Lyme disease isolate.

Department of Microbiology, University of Minnesota, Minneapolis 55455.

ABSTRACT

The DNA of the first northcentral United States human Lyme disease isolate, Borrellia burgdorferi NCH-1, was characterized and compared with the DNAs of nine other B. burgdorferi isolates. Strain NCH-1 was isolated in August 1989 from a human skin biopsy specimen. DNA was analyzed by pulsed-field gel electrophoresis and restriction endonuclease analysis. Contour-clamped homogeneous electric field pulsed-field gel electrophoresis of in situ-lysed cells was performed to compare the plasmid profiles of the various isolates. The plasmid profile of isolate NCH-1, which included five plasmids of approximately 69, 42, 38, 32, and 23 kb, could be distinguished from those of the other isolates examined. The DNA profile of NCH-1 was most similar to those of strain 297 (human cerebrospinal fluid isolate, Connecticut) and strain PAL (human erythema migrans isolate, New York) and most dissimilar from those of strain P/Gau (human erythema migrans isolate, Germany) and strain IPF (Ixodes persulcatus tick isolate, Japan). These results indicate that genetic diversity exists among B. burgdorferi strains isolated from different geographical areas.

This article has been cited by other articles:

• Revel, A. T., Blevins, J. S., Almazan, C., Neil, L., Kocan, K. M., de la Fuente, J., Hagman, K. E., Norgard, M. V. (2005). bptA (bbe16) is essential for the persistence of the Lyme disease spirochete, Borrelia burgdorferi, in its natural tick vector. Proc. Natl. Acad. Sci. USA 102: 6972-6977 [Abstract] [Full Text]  
• Blevins, J. S., Revel, A. T., Caimano, M. J., Yang, X. F., Richardson, J. A., Hagman, K. E., Norgard, M. V. (2004). The luxS Gene Is Not Required for Borrelia burgdorferi Tick Colonization, Transmission to a Mammalian Host, or Induction of Disease. Infect. Immun. 72: 4864-4867 [Abstract] [Full Text]  
• Yang, X. F., Alani, S. M., Norgard, M. V. (2003). The response regulator Rrp2 is essential for the expression of major membrane lipoproteins in Borrelia burgdorferi. Proc. Natl. Acad. Sci. USA 100: 11001-11006 [Abstract] [Full Text]  
• Yang, X. F., Hubner, A., Popova, T. G., Hagman, K. E., Norgard, M. V. (2003). Regulation of Expression of the Paralogous Mlp Family in Borrelia burgdorferi. Infect. Immun. 71: 5012-5020 [Abstract] [Full Text]  
• Hubner, A., Revel, A. T., Nolen, D. M., Hagman, K. E., Norgard, M. V. (2003). Expression of a luxS Gene Is Not Required for Borrelia burgdorferi Infection of Mice via Needle Inoculation. Infect. Immun. 71: 2892-2896 [Abstract] [Full Text]  
• Miller, J. C., Stevenson, B. (2003). Immunological and genetic characterization of Borrelia burgdorferi BapA and EppA proteins. Microbiology 149: 1113-1125 [Abstract] [Full Text]  
• Hubner, A., Yang, X., Nolen, D. M., Popova, T. G., Cabello, F. C., Norgard, M. V. (2001). Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway. Proc. Natl. Acad. Sci. USA 98: 12724-12729 [Abstract] [Full Text]  
• Yang, X., Popova, T. G., Goldberg, M. S., Norgard, M. V. (2001). Influence of Cultivation Media on Genetic Regulatory Patterns in Borrelia burgdorferi. Infect. Immun. 69: 4159-4163 [Abstract] [Full Text]  
• Felz, M. W., Chandler, F. W. Jr, Oliver, J. H. Jr, Rahn, D. W., Schriefer, M. E. (1999). Solitary Erythema Migrans in Georgia and South Carolina. Arch Dermatol 135: 1317-1326 [Abstract] [Full Text]