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J Clin Microbiol. 1992 March; 30(3): 732-734

Development of an immunoassay using recombinant maltose-binding protein-STa fusions for quantitating antibody responses against STa, the heat-stable enterotoxin of Escherichia coli.

R Aitken and T R Hirst

Department of Genetics, University of Leicester, United Kingdom.

ABSTRACT

A set of fusion proteins containing heat-stable enterotoxin (STa) and maltose-binding protein were engineered. These molecules were readily purified and used as solid-phase antigens in an enzyme-linked immunosorbent assay to monitor anti-STa responses in mice immunized with a recombinant vaccine composed of STa and the B subunit of heat-labile enterotoxin.


J Clin Microbiol. 1992 March; 30(3): 732-734




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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1992 by the American Society for Microbiology. All rights reserved.