This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, J T Articles by Chen, D S Search for Related Content PubMed PubMed Citation Articles by Wang, J T Articles by Chen, D S

 Previous Article  |  Next Article 

J Clin Microbiol. 1992 March; 30(3): 750-753

Effects of anticoagulants and storage of blood samples on efficacy of the polymerase chain reaction assay for hepatitis C virus.

Department of Internal Medicine, National Taiwan University Hospital, Taipei, Republic of China.

ABSTRACT

Blood samples from 11 patients with posttransfusion hepatitis C virus infection were collected. Each sample was divided into three fractions to obtain sera, sodium-citrated plasma, and heparinized plasma and then tested for HCV RNA by a nested polymerase chain reaction (PCR). Of them, eight sodium-citrated plasma samples, seven serum samples, and no heparinized plasma samples were PCR positive. Eight PCR-positive sodium-citrated plasma samples were exposed to different physical conditions and semiquantified for HCV RNA after serial dilutions. Samples stored at -70 degrees C showed the best preservation of HCV RNA, and storage at the other conditions resulted in only minimal loss of the PCR signal. Therefore, serum or sodium-citrated plasma specimens are satisfactory for detecting HCV RNA by PCR, but heparinized blood specimens are not.

This article has been cited by other articles:

• Al-Soud, W. A., Rådström, P. (2001). Purification and Characterization of PCR-Inhibitory Components in Blood Cells. J. Clin. Microbiol. 39: 485-493 [Abstract] [Full Text]  
• Dufour, D. R., Lott, J. A., Nolte, F. S., Gretch, D. R., Koff, R. S., Seeff, L. B. (2000). Diagnosis and Monitoring of Hepatic Injury. I. Performance Characteristics of Laboratory Tests. Clin. Chem. 46: 2027-2049 [Abstract] [Full Text]  
• Krajden, M., Minor, J. M., Rifkin, O., Comanor, L. (1999). Effect of Multiple Freeze-Thaw Cycles on Hepatitis B Virus DNA and Hepatitis C Virus RNA Quantification as Measured with Branched-DNA Technology. J. Clin. Microbiol. 37: 1683-1686 [Abstract] [Full Text]  
• Krajden, M., Comanor, L., Rifkin, O., Grigoriew, A., Minor, J. M., Kapke, G. F. (1998). Assessment of Hepatitis B Virus DNA Stability in Serum by the Chiron Quantiplex Branched-DNA Assay. J. Clin. Microbiol. 36: 382-386 [Abstract] [Full Text]  
• Parravicini, C., Lauri, E., Baldini, L., Neri, A., Poli, F., Sirchia, G., Moroni, M., Galli, M., Corbellino;, M., Masood, R., Zheng, T., Tulpule, A., Arora, N., Chatlynne, L., Handy, M., Whitman Jr., J., Kaplan, M., Dosik, M., Ablashi, D. V., Gill;, P. S., Whitby, D., Boshoff, C., Luppi, M., Torelli;, G., Cottoni, F., Uccini;, S., Brousset, P., Meggetto, F., Attal, M., Delsol;, G., Rettig, M. B., Sun, J. W. R., Berenson;, R. A. J. (1997). Kaposi's Sarcoma-Associated Herpesvirus Infection and Multiple Myeloma. Science 278: 1969-1973 [Full Text]  
• Ljubimova, J. Y., Petrovic, L. M., Wilson, S. E., Geller, S. A., Demetriou, A. A. (1997). Expression of HGF, Its Receptor c-met, c-myc, and Albumin in Cirrhotic and Neoplastic Human Liver Tissue. J. Histochem. Cytochem. 45: 79-88 [Abstract] [Full Text]  
• Burckhardt, J (1994). Amplification of DNA from whole blood.. Genome Res 3: 239-243 [Abstract]  
• Morales, J. M., Andres, A., Campistol, J. M., Miller, J., Roth, D., Schiff, E. R., Candinas, D., Joller-Jemelka, H. I., Largiader, F., Pereira, B. J.G., Levey, A. S. (1993). Hepatitis C Virus and Organ Transplantation. NEJM 328: 511-513 [Full Text]