This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ross, B C Articles by Dwyer, B Search for Related Content PubMed PubMed Citation Articles by Ross, B C Articles by Dwyer, B

Molecular cloning of a highly repeated DNA element from Mycobacterium tuberculosis and its use as an epidemiological tool.

Clinical Pathology Laboratory, Fairfield Hospital, Victoria, Australia.

ABSTRACT

In order to develop a technique for distinguishing between isolates of Mycobacterium tuberculosis, we cloned two hypervariable DNA fragments from NdeII-digested genomic DNA. The cloned DNA fragments of 3.8 and 4.7 kb were found to contain the same repetitive element, which was different from previously characterized repetitive elements. It is present in at least 30 copies per genome and is distributed among mycobacterial species other than those of the tuberculosis complex, including M. kansaii, M. gastri, and M. szulgai. When used as a probe on restriction enzyme-digested DNA, it can distinguish between strains from unrelated cases of tuberculosis while demonstrating identical banding patterns for isolates from epidemiologically related cases.

This article has been cited by other articles:

• Mathema, B., Kurepina, N. E., Bifani, P. J., Kreiswirth, B. N. (2006). Molecular Epidemiology of Tuberculosis: Current Insights. Clin. Microbiol. Rev. 19: 658-685 [Abstract] [Full Text]  
• Srivastava, R., Kumar, D., Waskar, M. N., Sharma, M., Katoch, V. M., Srivastava, B. S. (2006). Identification of a repetitive sequence belonging to a PPE gene of Mycobacterium tuberculosis and its use in diagnosis of tuberculosis.. J Med Microbiol 55: 1071-1077 [Abstract] [Full Text]  
• Dheenadhayalan, V., Delogu, G., Sanguinetti, M., Fadda, G., Brennan, M. J. (2006). Variable Expression Patterns of Mycobacterium tuberculosis PE_PGRS Genes: Evidence that PE_PGRS16 and PE_PGRS26 Are Inversely Regulated In Vivo.. J. Bacteriol. 188: 3721-3725 [Abstract] [Full Text]  
• Talarico, S., Cave, M. D., Marrs, C. F., Foxman, B., Zhang, L., Yang, Z. (2005). Variation of the Mycobacterium tuberculosis PE_PGRS33 Gene among Clinical Isolates. J. Clin. Microbiol. 43: 4954-4960 [Abstract] [Full Text]  
• Smittipat, N., Billamas, P., Palittapongarnpim, M., Thong-On, A., Temu, M. M., Thanakijcharoen, P., Karnkawinpong, O., Palittapongarnpim, P. (2005). Polymorphism of Variable-Number Tandem Repeats at Multiple Loci in Mycobacterium tuberculosis. J. Clin. Microbiol. 43: 5034-5043 [Abstract] [Full Text]  
• Otsuka, Y., Parniewski, P., Zwolska, Z., Kai, M., Fujino, T., Kirikae, F., Toyota, E., Kudo, K., Kuratsuji, T., Kirikae, T. (2004). Characterization of a Trinucleotide Repeat Sequence (CGG)5 and Potential Use in Restriction Fragment Length Polymorphism Typing of Mycobacterium tuberculosis. J. Clin. Microbiol. 42: 3538-3548 [Abstract] [Full Text]  
• Hirsh, A. E., Tsolaki, A. G., DeRiemer, K., Feldman, M. W., Small, P. M. (2004). From the Cover: Stable association between strains of Mycobacterium tuberculosis and their human host populations. Proc. Natl. Acad. Sci. USA 101: 4871-4876 [Abstract] [Full Text]  
• Mostowy, S., Cousins, D., Behr, M. A. (2004). Genomic Interrogation of the Dassie Bacillus Reveals It as a Unique RD1 Mutant within the Mycobacterium tuberculosis Complex. J. Bacteriol. 186: 104-109 [Abstract] [Full Text]  
• Douglas, J. T., Qian, L., Montoya, J. C., Musser, J. M., Van Embden, J. D. A., Van Soolingen, D., Kremer, K. (2003). Characterization of the Manila Family of Mycobacterium tuberculosis. J. Clin. Microbiol. 41: 2723-2726 [Abstract] [Full Text]  
• Maguire, H, Dale, J W, McHugh, T D, Butcher, P D, Gillespie, S H, Costetsos, A, Al-Ghusein, H, Holland, R, Dickens, A, Marston, L, Wilson, P, Pitman, R, Strachan, D, Drobniewski, F A, Banerjee, D K (2003). Molecular epidemiology of tuberculosis in London 1995-7 showing low rate of active transmission. Mol. Pathol. 56: 121-126 [Abstract] [Full Text]  
• Gutacker, M. M., Smoot, J. C., Migliaccio, C. A. L., Ricklefs, S. M., Hua, S., Cousins, D. V., Graviss, E. A., Shashkina, E., Kreiswirth, B. N., Musser, J. M. (2002). Genome-Wide Analysis of Synonymous Single Nucleotide Polymorphisms in Mycobacterium tuberculosis Complex Organisms: Resolution of Genetic Relationships Among Closely Related Microbial Strains. Genetics 162: 1533-1543 [Abstract] [Full Text]  
• Weis, S. E., Pogoda, J. M., Yang, Z., Cave, M. D., Wallace, C., Kelley, M., Barnes, P. F. (2002). Transmission Dynamics of Tuberculosis in Tarrant County, Texas. Am. J. Respir. Crit. Care Med. 166: 36-42 [Abstract] [Full Text]  
• Maguire, H, Dale, J W, McHugh, T D, Butcher, P D, Gillespie, S H, Costetsos, A, Al-Ghusein, H, Holland, R, Dickens, A, Marston, L, Wilson, P, Pitman, R, Strachan, D, Drobniewski, F A, Banerjee, D K (2002). Molecular epidemiology of tuberculosis in London 1995-7 showing low rate of active transmission. Thorax 57: 617-622 [Abstract] [Full Text]  
• Roring, S., Scott, A., Brittain, D., Walker, I., Hewinson, G., Neill, S., Skuce, R. (2002). Development of Variable-Number Tandem Repeat Typing of Mycobacterium bovis: Comparison of Results with Those Obtained by Using Existing Exact Tandem Repeats and Spoligotyping. J. Clin. Microbiol. 40: 2126-2133 [Abstract] [Full Text]  
• Borgdorff, M. W., Nagelkerke, N. J. D., de Haas, P. E. W., van Soolingen, D. (2001). Transmission of Mycobacterium tuberculosis Depending on the Age and Sex of Source Cases. Am J Epidemiol 154: 934-943 [Abstract] [Full Text]  
• Golub, J. E., Cronin, W. A., Obasanjo, O. O., Coggin, W., Moore, K., Pope, D. S., Thompson, D., Sterling, T. R., Harrington, S., Bishai, W. R., Chaisson, R. E. (2001). Transmission of Mycobacterium tuberculosis Through Casual Contact With an Infectious Case. Arch Intern Med 161: 2254-2258 [Abstract] [Full Text]  
• Barlow, R. E. L., Gascoyne-Binzi, D. M., Gillespie, S. H., Dickens, A., Qamer, S., Hawkey, P. M. (2001). Comparison of Variable Number Tandem Repeat and IS6110-Restriction Fragment Length Polymorphism Analyses for Discrimination of High- and Low-Copy-Number IS6110 Mycobacterium tuberculosis Isolates. J. Clin. Microbiol. 39: 2453-2457 [Abstract] [Full Text]  
• Wendel, K. A., Alwood, K. S., Gachuhi, R., Chaisson, R. E., Bishai, W. R., Sterling, T. R. (2001). Paradoxical Worsening of Tuberculosis in HIV-Infected Persons. Chest 120: 193-197 [Abstract] [Full Text]  
• Yang, Z. H., Bates, J. H., Eisenach, K. D., Cave, M. D. (2001). Secondary Typing of Mycobacterium tuberculosis Isolates with Matching IS6110 Fingerprints from Different Geographic Regions of the United States. J. Clin. Microbiol. 39: 1691-1695 [Abstract] [Full Text]  
• Ramakrishnan, L., Federspiel, N. A., Falkow, S. (2000). Granuloma-Specific Expression of Mycobacterium Virulence Proteins from the Glycine-Rich PE-PGRS Family. Science 288: 1436-1439 [Abstract] [Full Text]  
• O'Brien, R., Flynn, O., Costello, E., O'Grady, D., Rogers, M. (2000). Identification of a Novel DNA Probe for Strain Typing Mycobacterium bovis by Restriction Fragment Length Polymorphism Analysis. J. Clin. Microbiol. 38: 1723-1730 [Abstract] [Full Text]  
• Park, Y.-K., Bai, G.-H., Kim, S.-J. (2000). Restriction Fragment Length Polymorphism Analysis of Mycobacterium tuberculosis Isolated from Countries in the Western Pacific Region. J. Clin. Microbiol. 38: 191-197 [Abstract] [Full Text]  
• Bifani, P. J., Mathema, B., Liu, Z., Moghazeh, S. L., Shopsin, B., Tempalski, B., Driscoll, J., Frothingham, R., Musser, J. M., Alcabes, P., Kreiswirth, B. N. (1999). Identification of a W Variant Outbreak of Mycobacterium tuberculosis via Population-Based Molecular Epidemiology. JAMA 282: 2321-2327 [Abstract] [Full Text]  
• Espitia, C., Laclette, J. P., Mondragón- Palomino, M., Amador, A., Campuzano, J., Martens, A., Singh, M., Cicero, R., Zhang, Y., Moreno, C. (1999). The PE-PGRS glycine-rich proteins of Mycobacterium tuberculosis : a new family of fibronectin-binding proteins?. Microbiology 145: 3487-3495 [Abstract] [Full Text]  
• Costello, E., O'Grady, D., Flynn, O., O'Brien, R., Rogers, M., Quigley, F., Egan, J., Griffin, J. (1999). Study of Restriction Fragment Length Polymorphism Analysis and Spoligotyping for Epidemiological Investigation of Mycobacterium bovis Infection. J. Clin. Microbiol. 37: 3217-3222 [Abstract] [Full Text]  
• Portugal, I., Maia, S., Moniz-Pereira, J. (1999). Discrimination of Multidrug-Resistant Mycobacterium tuberculosis IS6110 Fingerprint Subclusters by rpoB Gene Mutation Analysis. J. Clin. Microbiol. 37: 3022-3024 [Abstract] [Full Text]  
• Kremer, K., van Soolingen, D., Frothingham, R., Haas, W. H., Hermans, P. W. M., Martín, C., Palittapongarnpim, P., Plikaytis, B. B., Riley, L. W., Yakrus, M. A., Musser, J. M., van Embden, J. D. A. (1999). Comparison of Methods Based on Different Molecular Epidemiological Markers for Typing of Mycobacterium tuberculosis Complex Strains: Interlaboratory Study of Discriminatory Power and Reproducibility. J. Clin. Microbiol. 37: 2607-2618 [Abstract] [Full Text]  
• Jasmer, R. M., Hahn, J. A., Small, P. M., Daley, C. L., Behr, M. A., Moss, A. R., Creasman, J. M., Schecter, G. F., Paz, E. A., Hopewell, P. C. (1999). A Molecular Epidemiologic Analysis of Tuberculosis Trends in San Francisco, 1991-1997. ANN INTERN MED 130: 971-978 [Abstract] [Full Text]  
• Zumárraga, M. J., Martin, C., Samper, S., Alito, A., Latini, O., Bigi, F., Roxo, E., Cicuta, M. E., Errico, F., Ramos, M. C., Cataldi, A., van Soolingen, D., Romano, M. I. (1999). Usefulness of Spoligotyping in Molecular Epidemiology of Mycobacterium bovis-Related Infections in South America. J. Clin. Microbiol. 37: 296-303 [Abstract] [Full Text]  
• Bishai, W. R., Graham, N. M. H., Harrington, S., Pope, D. S., Hooper, N., Astemborski, J., Sheely, L., Vlahov, D., Glass, G. E., Chaisson, R. E. (1998). Molecular and Geographic Patterns of Tuberculosis Transmission After 15 Years of Directly Observed Therapy. JAMA 280: 1679-1684 [Abstract] [Full Text]  
• van Belkum, A., Scherer, S., van Alphen, L., Verbrugh, H. (1998). Short-Sequence DNA Repeats in Prokaryotic Genomes. Microbiol. Mol. Biol. Rev. 62: 275-293 [Abstract] [Full Text]  
• Fang, Z., Morrison, N., Watt, B., Doig, C., Forbes, K. J. (1998). IS6110 Transposition and Evolutionary Scenario of the Direct Repeat Locus in a Group of Closely Related Mycobacterium tuberculosis Strains. J. Bacteriol. 180: 2102-2109 [Abstract] [Full Text]  
• Vitale, F., Capra, G., Maxia, L., Reale, S., Vesco, G., Caracappa, S. (1998). Detection of Mycobacterium tuberculosis Complex in Cattle by PCR Using Milk, Lymph Node Aspirates, and Nasal Swabs. J. Clin. Microbiol. 36: 1050-1055 [Abstract] [Full Text]  
• Sola, C., Horgen, L., Maïsetti, J., Devallois, A., Goh, K. S., Rastogi, N. (1998). Spoligotyping Followed by Double-Repetitive-Element PCR as Rapid Alternative to IS6110 Fingerprinting for Epidemiological Studies of Tuberculosis. J. Clin. Microbiol. 36: 1122-1124 [Abstract] [Full Text]  
• Gutiérrez, M. C., Vincent, V., Aubert, D., Bizet, J., Gaillot, O., Lebrun, L., Le Pendeven, C., Le Pennec, M. P., Mathieu, D., Offredo, C., Pangon, B., Pierre-Audigier, C. (1998). Molecular Fingerprinting of Mycobacterium tuberculosis and Risk Factors for Tuberculosis Transmission in Paris, France, and Surrounding Area. J. Clin. Microbiol. 36: 486-492 [Abstract] [Full Text]  
• Cousins, D, Williams, S, Liebana, E, Aranaz, A, Bunschoten, A, Van Embden, J, Ellis, T (1998). Evaluation of four DNA typing techniques in epidemiological investigations of bovine tuberculosis [In Process Citation]. J. Clin. Microbiol. 36: 168-178 [Abstract] [Full Text]  
• JONES, B. E., RYU, R., YANG, Z., CAVE, M. D., POGODA, J. M., OTAYA, M., BARNES, P. F. (1997). Chest Radiographic Findings in Patients with Tuberculosis with Recent or Remote Infection. Am. J. Respir. Crit. Care Med. 156: 1270-1273 [Abstract] [Full Text]  
• Bishai, W. R., Graham, N. M.H., Harrington, S., Page, C., Moore-Rice, K., Hooper, N., Chaisson, R. E. (1996). Rifampin-Resistant Tuberculosis in a Patient Receiving Rifabutin Prophylaxis. NEJM 334: 1573-1576 [Full Text]