This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vera-Cabrera, L Articles by Rodriguez, M A Search for Related Content PubMed PubMed Citation Articles by Vera-Cabrera, L Articles by Rodriguez, M A

Isolation and purification of two immunodominant antigens from Nocardia brasiliensis.

Departamento de Inmunologia, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico.

ABSTRACT

Two immunogenic proteins from a crude extract of Nocardia brasiliensis were purified to homogeneity. A 61-kDa protein (P61) was isolated from a 50% ammonium sulfate precipitate in two steps. Initially, P61 was obtained by electroelution in a 10% nondenatured preparative polyacrylamide gel electrophoresis (PAGE). In a second step, the eluate from the nondenatured gel was run in a 12% sodium dodecyl sulfate (SDS) preparative polyacrylamide gel. After elution, a single band was demonstrated by SDS-PAGE and Western blot (immunoblot). Also, a 24-kDa immunogenic protein (P24) was isolated by gel filtration in a Sephadex G-100 column and then by electroelution in a 12% nondenatured polyacrylamide gel. In a previous paper, we showed by Western blot assays that these proteins are recognized by the sera of mycetoma patients and not by sera from mycobacterial-infected or healthy individuals. We consider these proteins to be good candidates for the study of the host-parasite relationship in nocardial infections. The possible clinical application of these purified antigens in a serological diagnosis is discussed.

This article has been cited by other articles:

• Salinas-Carmona, M. C., Perez-Rivera, I. (2004). Humoral Immunity through Immunoglobulin M Protects Mice from an Experimental Actinomycetoma Infection by Nocardia brasiliensis. Infect. Immun. 72: 5597-5604 [Abstract] [Full Text]  
• Vera-Cabrera, L., Johnson, W. M., Welsh, O., Resendiz-Uresti, F. L., Salinas-Carmona, M. C. (1999). Distribution of a Nocardia brasiliensis Catalase Gene Fragment in Members of the Genera Nocardia, Gordona, and Rhodococcus. J. Clin. Microbiol. 37: 1971-1976 [Abstract] [Full Text]  
• Salinas-Carmona, M. C., Torres-Lopez, E., Ramos, A. I., Licon-Trillo, A., Gonzalez-Spencer, D. (1999). Immune Response to Nocardia brasiliensis Antigens in an Experimental Model of Actinomycetoma in BALB/c Mice. Infect. Immun. 67: 2428-2432 [Abstract] [Full Text]  
• Sugar, A. M., Mattia, A. R. (1994). Case 28-1994- A 51-Year-Old Man with a Nonhealing Finger Wound and Regional Lymphadenopathy. NEJM 331: 181-187 [Full Text]